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Anti-Butyryl-Histone H2B (Lys20) Mouse mAb
Catalog Number: PTM-316
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: H2BK20bu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H2BK20bu
UniProt ID

P62807

Immunogen Butyrylated human histone H2B (Lys20) peptide
MW (kDa) 14
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:50 - 1:200 Human, Mouse
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H2B at lysine 20 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 60 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) Hela + sodium butyrate (30mM, 4 hours)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 14 kDa
Observed band size: 14 kDa
IHC-P

Tissue: Human spleen
Section type: Formalin fixed & Paraffin – embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-316

Tissue: Mouse cerebrum
Section type: Formalin fixed & Paraffin – embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-316

Research Use

For research use only, not for use in diagnostic procedures.

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    Host
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    Conjugate
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    Reactivity
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    Applications：
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    Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
    Catalog #
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    Host
    Goat
    Clonality
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    Conjugate
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    Reactivity
    Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
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