中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-Butyryl-Histone H3 (Lys14) Rabbit mAb
Catalog Number: PTM-334RM
$ 435

Clone Number: PA-039-24BH0L0

Host: Rabbit Clonality: Monoclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: H3K14bu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K14bu
UniProt ID

P68431

Immunogen
MW (kDa) 15
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500-1:2000 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

H3 is core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of histones marks active promoters and competes with histone acetylation, which occurs during late

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Immunogen peptide quantity: 1 ng, 4 ng, 16ng, 64 ng
Exposure time: 60 s
The list of peptides is included in the table below:
WB

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L(HRP)
Lysate: (-): MCF-7+serum starvation (14 h), (+): MCF-7+ sodium butyrate (50 mM, 24 h) +trichostain A (500 ng/ml, 4 h)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L(HRP)
Lysate: (-): HeLa, (+): HeLa+ sodiumbutyrate(30 mM, 4 h)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L(HRP)
Lysate: 1: Neuro-2a, 2: BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Research Use

For research use only, not for use in diagnostic procedures.

Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.