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Recombinant Rabbit Monoclonal
Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb
Catalog Number: PTM-306RM
$ 420

Clone Number: PA-177-23

Host: Rabbit Clonality: Monoclonal

Applications： WB

Reactivity: Human

Synonyms: H3K18bu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K18bu
UniProt ID

P68431

Immunogen Butyrylated human histone H3 (Lys18) peptide
MW (kDa) 15
Specificity Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb detects histone H3 only when it is butyrylated at Lys18. 
Product Usage Information
Applications Dilution Recommended Species
WB 1:500-1:2000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation and propionylation, is a newly identified reversible modification controlling chromosome structure and gene transcription. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Peptide amount: 1-2: 1ng, 4ng, 16 ng, 64 ng; 3-9: 64 ng
Exposure time: 30 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 h)
Protein loading amount: 20 μg
Exposure time: 30 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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