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Recombinant Rabbit Monoclonal
Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb
Catalog Number: PTM-338RM
$ 420

Clone Number: PA-O0801

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IP ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H3K18bu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K18bu
UniProt ID

P68431

Immunogen Butyrylated human histone H3 (Lys18) peptide
MW (kDa) 15
Specificity Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb detects histone H3 only when it is butyrylated at Lys18. 
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Human, Mouse, Rat
ChIP 6ug antibody/100ug chromatin Human
IP 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary Ab incubation: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1-2: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 60 s The list of peptides is included in the table below: No. Peptide No. Peptide Lane 1 H3K18bu Lane 2 H3K18cr Lane 3 H3K18pr Lane 4 H3K18ac Lane 5 H3K18hib Lane 6 H3K18bhb Lane 7 H3K14bu Lane 8 H3K23bu Lane 9 H3K18un
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): HeLa, (+): HeLa + Sodium Butyrate (5mM, 24h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): NIH-3T3, (+): NIH-3T3 + Sodium Butyrate (30mM, 4h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): C6, (+): C6 + Sodium Butyrate (30mM, 4h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
IP

IP of HeLa+SBA(5mM, 24h) cells extracts IP Ab incubation: PTM-338RM, 4°C overnight, 1:100 dilution WB Primary Ab incubation: PTM-338RM, room temperature 2h, 1: 1000 dilution Secondary Ab: Anti-Rabbit IgG for IP (HRP) Blocking buffer: 5% NFDM/TBST Dilution buffer: 5% NFDM/TBST 1: 5% Input 2: IP with PTM-338RM 3: IP with Rabbit monoclonal IgG Isotype Observed MW: 15 kDa Exposure time: 60 s
ChIP

Cell type: HeLa+SBA(5mM, 24h) Cross-linking conditions: no cross-linking Amount of chromatin per IP: 100 μg Amount of Ab per IP: 6 ug Beads type and amount per IP: 50 μl of Protein A MagBeads Comment: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDP-CDS, RPL30, TuBBP10, Foxo3a-promoter, LDHA-promoter, LDHA-CDS and MYT1-JBC. Data are presented as enrichment of each sample relative to the total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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