Applications：
WB
IHC-P
ICC/IF
FC
IAP
IP
ChIP
mIHC-P
Clone Number: /
Host: Rabbit Clonality: Polyclonal
Applications： WB ICC/IF
Reactivity: Human, Mouse, Rat
Synonyms: H3K18bu
Shipping: Ambient temperature
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| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | H3K18bu |
| UniProt ID | |
| Immunogen | Butyrylated human histone H3 (Lys18) peptide |
| MW (kDa) | 15 |
| Specificity | Anti-Butyryl-Histone H3 (Lys18) Rabbit pAb detects histone H3 only when it is butyrylated at Lys18. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:2000 | Human, Mouse, Rat |
| ICC/IF | 1:50 | Human |
| Purity | Protein A and immunogen affinity purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation
Cellular location
Nucleus
| Dot Blot | |
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Blocking buffer: 5% NFDM/TBST |
| WB | |
|---|---|
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Blocking buffer: 5% NFDM/TBST |
| ICC/IF | |
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Cell line: (A) HeLa, (B) HeLa+ SBA (5mM, 24 hours) |
Research Use
For research use only, not for use in diagnostic procedures.
