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Anti-Butyryl-Histone H3 (Lys23) Mouse mAb
Catalog Number: PTM-307
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: H3K23bu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K23bu
UniProt ID

P68431

Immunogen Butyrylated human histone H3 (Lys23) peptide
MW (kDa) 15
Specificity Anti-Butyryl-Histone H3 (Lys23) Mouse mAb detects histone H3 only when it is butyrylated at Lys23. 
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:50 - 1:200 Human, Mouse
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K23 butyryl. Lane 2: H3K18 acetyl.
Lane 3: H3K18 propionyl. Lane 4: H3K18 butyryl.
Lane 5: H3K18 crotonyl. Lane 6: H3K18 Monomethyl.
Lane 7: H3K23 acetyl. Lane 8: H3K23 propionyl.
Lane 9: H3K23 crotonyl. Lane 10: H3K23 Monomethyl.
Lane 11: H3K27 acetyl. Lane 12: H3K27 crotonyl.
Lane 13: H3K27 Monomethyl. Lane 14: H3K27 Dimethyl.
Lane 15: H3K27 Trimethyl. Lane 16: H3K23 unmodified.
WB

Lysates: (-): HeLa cells; (+): HeLa cells treated with 30 mM sodium butyrate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
IHC-P

Tissue: Human spleen
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-307.

Tissue: Mouse cerebrum
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-307.

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 160
    Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-7168
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (3)
  • Butyrylation Meets Adipogenesis-Probed by a p300-Catalyzed Acylation-Specific Small Molecule Inhibitor: Implication in Anti-obesity Therapy
    Year 2022
    Journal JOURNAL OF MEDICINAL CHEMISTRY
    Authors Aditya Bhattacharya, et al.
    Applications Unspecified
    Reactivity
  • Deficient histone H3 propionylation by BRPF1-KAT6 complexes in neurodevelopmental disorders and cancer
    Year 2020
    Journal Science Advances
    Authors Kezhi Yan Justine Rousseau, et al.
    Applications Unspecified
    Reactivity
  • Histone acylation marks respond to metabolic perturbations and enable cellular adaptation
    Year 2020
    Journal EXPERIMENTAL AND MOLECULAR MEDICINE
    Authors Jo Chanhee, et al.
    Applications Unspecified
    Reactivity
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