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Anti-Butyryl-Histone H3 (Lys27) Mouse mAb
Catalog Number: PTM-326
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: H3K27bu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K27bu
UniProt ID

P68431

Immunogen Butyrylated human histone H3 (Lys27) peptide
MW (kDa) 15
Specificity Anti-Butyryl-Histone H3 (Lys27) Mouse mAb detects histone H3 only when it is butyrylated at Lys27. 
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ICC/IF 1:50 Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 60 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
ICC/IF

Cell line:(A) HeLa, (B) HeLa+Sodium butyrate (30mM, 4hr)
Fixation:100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°Covernight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for PTM-326

Research Use

For research use only, not for use in diagnostic procedures.

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