中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Anti-Butyryl-Histone H3 (Lys9) Rabbit pAb
Catalog Number: PTM-305
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB ICC/IF ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H3K9bu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K9bu
UniProt ID

P68431

Immunogen Butyrylated human histone H3 (Lys9) peptide
MW (kDa) 15
Specificity Anti-Butyryl-Histone H3 (Lys9) Rabbit pAb detects histone H3 only when it is butyrylated at Lys9. 
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ICC/IF 1:50 Human
ChIP 12 μg per 5x106 cells Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. This modification is highly conserved across both prokaryotic and eukaryotic organisms. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K9 butyryl. Lane 2: H3K4 crotonyl.
Lane 3: H3K9 acetyl. Lane 4: H3K9 crotonyl.
Lane 5: H3K9 unmodified.
WB

Lysates: (-) HeLa cells; (+) HeLa cells treated with 30 mM sodium butyrate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 17 kDa
ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells treated with 5 mM sodium butyrate for 24 hours
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-305.
ChIP

Sample: HeLa cells + serum starvation (12 hours) + sodium butyryate (5 mM, 24 hours)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 4 μg, 12 μg
Beads type and amount per IP: 50 μL of Protein A/G MagBeads
Comment: The ChIP assay was performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH promoter, GAPDH CDS, RPL30 exon 3, FOXO3a-promoter, and FOXO3a-downstream regions. The data are presented as enrichment of each sample relative to total amount of input at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
Alternative Products Compatible Secondaries Isotype Control Writers/Readers/Erasers Related Products
  • View More Images（4）
    $ 420
    Anti-Butyryl-Histone H3 (Lys9) Mouse mAb
    Catalog #
    PTM-312
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 125
    Recombinant Rabbit Monoclonal
    Rabbit mAb IgG Isotype Control
    Catalog #
    PTM-5073
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 125
    Recombinant Rabbit Monoclonal
    Rabbit mAb IgG Isotype Control
    Catalog #
    PTM-6628
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（11）
    $ 320
    Recombinant Rabbit Monoclonal
    Anti-Acetyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-112RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（9）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-Crotonyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-516RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 320
    Recombinant Rabbit Monoclonal
    Anti-Monomethyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-7287
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 330
    Recombinant Rabbit Monoclonal
    Anti-Dimethyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-615RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（8）
    $ 320
    Recombinant Rabbit Monoclonal
    Anti-Trimethyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-616RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（3）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-β-Hydroxybutyryl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-1250RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（6）
    $ 435
    Recombinant Rabbit Monoclonal
    Anti-L-Lactyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-1419RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（6）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-Butyryllysine Rabbit mAb
    Catalog #
    PTM-301RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (2)
  • Histone butyrylation in the mouse intestine is mediated by the microbiota and associated with regulation of gene expression
    Year 2024
    Journal Nature Metabolism
    Authors Gates Leah A., et al.
    Applications Unspecified
    Reactivity
  • Butyrylation Meets Adipogenesis-Probed by a p300-Catalyzed Acylation-Specific Small Molecule Inhibitor: Implication in Anti-obesity Therapy
    Year 2022
    Journal JOURNAL OF MEDICINAL CHEMISTRY
    Authors Aditya Bhattacharya, et al.
    Applications Unspecified
    Reactivity
Service
Products
Support
About Us
© 2025 PTM BIO LLC, or its affiliates