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Recombinant Rabbit Monoclonal
Anti-Butyryllysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-302
$ 1,920

Clone Number: /

Host: / Clonality: /

Applications： IAP

Reactivity: All

Synonyms: Kbu

Product Size
500μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms Kbu
UniProt ID

/

Immunogen Butyrylated lysine peptides
MW (kDa) Multiple
Specificity With the immobilization of highly specific anti-butyryllysine antibody, the Anti-Butyryllysine Antibody Conjugated Agarose Beads selectively capture peptides/proteins bearing butyryllysine residues, but do not cross-reactwith the peptides/proteins bearing other structurally similar modified residues. This product has been well utilized to affinity purify peptides for global proteomic screening of lysine butyrylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Lysine butyrylation is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Lysine butyrylation may play a vital role in epigenetic modulation by impacting chromatin dynamics and lasticity, DNA transcriptional regulation and tumorigenesis, etc.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-Butyryllysine Rabbit pAb (PTM-301) on different acylation proteins.
Protein amount: 4 ng, 20 ng, 100 ng, 500 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of proteins used in the experiment is provided below.
Lane 1: Butyrylated BSA. Lane 2: Acetylated BSA.
Lane 3: Propionylated BSA. Lane 4: BSA.

Research Use

For research use only, not for use in diagnostic procedures.

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  • The rate of glycolysis quantitatively mediates specific histone acetylation sites
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