中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Anti-Butyryllysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-302
$ 1,920

Clone Number: /

Host: / Clonality: /

Applications： IAP

Reactivity: All

Synonyms: Kbu

Product Size
500 μl
Quantity

Shipping: Cold packs / Dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms Kbu
UniProt ID

/

Immunogen Butyrylated lysine peptides
MW (kDa) Multiple
Specificity With the immobilization of highly specific anti-butyryllysine antibody, the Anti-Butyryllysine Antibody Conjugated Agarose Beads selectively capture peptides/proteins bearing butyryllysine residues, but do not cross-reactwith the peptides/proteins bearing other structurally similar modified residues. This product has been well utilized to affinity purify peptides for global proteomic screening of lysine butyrylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Lysine butyrylation is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Lysine butyrylation may play a vital role in epigenetic modulation by impacting chromatin dynamics and lasticity, DNA transcriptional regulation and tumorigenesis, etc.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-Butyryllysine Rabbit pAb (PTM-301) on different acylation proteins.
Protein amount: 4 ng, 20 ng, 100 ng, 500 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of proteins used in the experiment is provided below.
Lane 1: Butyrylated BSA. Lane 2: Acetylated BSA.
Lane 3: Propionylated BSA. Lane 4: BSA.

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
Compatible Secondaries Isotype Control Writers/Readers/Erasers Related Products
  • View More Images（3）
    $ 280
    Recombinant
    Anti-SIRT1 Mouse mAb
    Catalog #
    PTM-5021
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 300
    Recombinant Rabbit Monoclonal
    Anti-Phospho-SIRT1 (Ser47) Rabbit mAb
    Catalog #
    PTM-6595
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 300
    Recombinant Rabbit Monoclonal
    Anti-Phospho-SIRT1 (Ser27) Rabbit mAb
    Catalog #
    PTM-6601
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 280
    Recombinant
    Anti-SIRT2 Mouse mAb
    Catalog #
    PTM-6043
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（6）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-Butyryllysine Rabbit mAb
    Catalog #
    PTM-301RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 1,800
    Anti-Acetyllysine Antibody Conjugated Agarose Beads
    Catalog #
    PTM-104
    Host
    /
    Clonality
    /
    Conjugate
    Agarose
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 1,920
    Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads
    Catalog #
    PTM-1104
    Host
    /
    Clonality
    /
    Conjugate
    Agarose
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 3,940
    Anti-L-Lactyllysine Antibody Conjugated Agarose Beads
    Catalog #
    PTM-1404
    Host
    Rabbit
    Clonality
    /
    Conjugate
    Agarose
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 1,920
    Anti-Succinyllysine Antibody Conjugated Agarose Beads
    Catalog #
    PTM-402
    Host
    /
    Clonality
    /
    Conjugate
    Agarose
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (4)
  • Dynamics and functional interplay of histone lysine butyrylation, crotonylation, and acetylation in rice under starvation and submergence
    Year 2018
    Journal GENOME BIOLOGY
    Authors Lu Yue, et al.
    Applications Unspecified
    Reactivity
  • Protein Acylation is a General Regulatory Mechanism in Biosynthetic Pathway of Acyl-CoA-Derived Natural Products
    Year 2018
    Journal Cell Chemical Biology
    Authors Jun-Yu Xu, et al.
    Applications Unspecified
    Reactivity
  • Protein Acetylation and Butyrylation Regulate the Phenotype and Metabolic Shifts of the Endospore-forming Clostridium acetobutylicum*
    Year 2018
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Jun-Yu Xu, et al.
    Applications Unspecified
    Reactivity
  • The rate of glycolysis quantitatively mediates specific histone acetylation sites
    Year 2015
    Journal Cancer & Metabolism
    Authors Cluntun Ahmad A, et al.
    Applications Unspecified
    Reactivity
Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.