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Anti-Butyryllysine Mouse mAb
Catalog Number: PTM-329
$ 435

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB ICC/IF

Reactivity: All

Synonyms: Kbu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kbu
UniProt ID

/

Immunogen Butyrylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Butyryllysine Mouse mAb detects proteins with Butyrylated lysine residues. This pan antibody recognizes Butyrylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 All
ICC/IF 1:100 Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Butyrylation of lysine, structurally similar to lysine acetylation and lysine propionylation, is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Given the facts that many lysine residues in histones and non-histone substrates, such as p53, p300/CBP, are butyrylated, lysine butyrylation may play a vital role in epigenetic modulation by impacting on chromatin dynamics and plasticity, DNA transcriptional regulation and tumorigenesis, etc.

Cellular location

/

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 100 ng
Exposure time: 60 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse liver
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: Multiple kDa
Observed MW: Multiple kDa
ICC/IF

Cell line: (A) HeLa, (B) HeLa + Sodium butyrate(5mM, 24hr)
Fixation: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:100
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for PTM-329

Research Use

For research use only, not for use in diagnostic procedures.

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References (1)
  • Short-chain fatty acids activate acetyltransferase p300
    Year 2021
    Journal eLife
    Authors Sydney P Thomas, et al.
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