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Recombinant Rabbit Monoclonal
Anti-Butyryllysine Rabbit mAb (BSA and Azide Free)
Catalog Number: PTM-337RM
$ 645

Clone Number: PSPTM-O0401

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: All

Synonyms: Kbu

Product Size
100 μg
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kbu
UniProt ID

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Immunogen Butyrylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Butyryllysine Rabbit mAb (BSA and Azide Free) detects proteins with Butyrylated lysine residues. This pan antibody recognizes Butyrylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 All
Properties
Purity Protein A purified
Constituents PBS
Storage Upon delivery centrifugate. Reconstitute with 100 μL distilled water (1.0 mg/mL after restoration). Aliquot and store at -80 °C. Avoid freeze / thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Butyrylation of lysine, structurally similar to lysine acetylation and lysine propionylation, is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Given the facts that many lysine residues in histones and non-histone substrates, such as p53, p300/CBP, are butyrylated, lysine butyrylation may play a vital role in epigenetic modulation by impacting on chromatin dynamics and plasticity, DNA transcriptional regulation and tumorigenesis, etc.

Cellular location

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Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Peptide quantity: 4 ng, 20 ng, 100 ng
Exposure time: 60 s
The list of peptides is included below:
Lane 1 Kbu library. Lane 2 Kac library.
Lane 3 Kpr library. Lane 4 Kcr library.
Lane 5 Khib library. Lane 6 Kbhb library.
Lane 7 Library control.

WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 4℃ overnight
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): HeLa, (+): HeLa+ Sodium Butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: Multiple kDa
Observed MW: Multiple kDa

Research Use

For research use only, not for use in diagnostic procedures.

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