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Anti-Butyryllysine Rabbit pAb
Catalog Number: PTM-301
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB

Reactivity: All

Synonyms: Kbu

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kbu
UniProt ID

/

Immunogen Butyrylated lysine peptides
MW (kDa) Multiple
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Butyrylation of lysine, structurally similar to lysine acetylation and lysine propionylation, is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation are widely distributed in wide ranges of prokaryotic and eukaryotic histones and non-histone substrates. Lysine butyrylation may play a vital role in the regulation of transcriptional regulation, tumorigenesis, chromatin dynamics, and plasticity.

Cellular location

/

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 4 ng, 20 ng, 100 ng, 500 ng
Exposure time: 60 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: lane A: PTM301+unmodified BSA peptides, lane B: PTM301+ butyrylated BSA peptides, 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HeLa
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: Multiple kDa
Observed band size: Multiple kDa

Research Use

For research use only, not for use in diagnostic procedures.

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References (17)
  • Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner
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    Authors Liao Long, et al.
    Applications Unspecified
    Reactivity
  • Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease
    Year 2022
    Journal JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
    Authors Lin Dang, et al.
    Applications Unspecified
    Reactivity
  • Butyrylation Meets Adipogenesis-Probed by a p300-Catalyzed Acylation-Specific Small Molecule Inhibitor: Implication in Anti-obesity Therapy
    Year 2022
    Journal JOURNAL OF MEDICINAL CHEMISTRY
    Authors Aditya Bhattacharya, et al.
    Applications Unspecified
    Reactivity
  • Histone crotonylation promotes mesoendodermal commitment of human embryonic stem cells
    Year 2021
    Journal Cell Stem Cell
    Authors Yi Fang, et al.
    Applications Unspecified
    Reactivity
  • Identification of lysine isobutyrylation as a new histone modification mark
    Year 2021
    Journal NUCLEIC ACIDS RESEARCH
    Authors Zhu Zhesi, et al.
    Applications Unspecified
    Reactivity
  • Epigenetics Identifier screens reveal regulators of chromatin acylation and limited specificity of acylation antibodies
    Year 2021
    Journal Scientific Reports
    Authors Kollenstart, et al.
    Applications Unspecified
    Reactivity
  • Protein Hyperacylation Links Mitochondrial Dysfunction with Nuclear Organization
    Year 2020
    Journal MOLECULAR CELL
    Authors Smestad John, et al.
    Applications Unspecified
    Reactivity
  • Starvation promotes histone lysine butyrylation in the liver of male but not female mice
    Year 2020
    Journal GENE
    Authors Afsaneh Goudarzi, et al.
    Applications Unspecified
    Reactivity
  • Characterization of the Sperm Proteome and Reproductive Outcomes with in Vitro, Fertilization after a Reduction in Male Ejaculatory Abstinence Period *
    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Zi-Qi Shen, et al.
    Applications Unspecified
    Reactivity
  • Genome-wide Profiling of Histone Lysine Butyrylation Reveals its Role in the Positive Regulation of Gene Transcription in Rice
    Year 2019
    Journal Rice
    Authors Liu Shuai, et al.
    Applications Unspecified
    Reactivity
  • Dynamics and functional interplay of histone lysine butyrylation, crotonylation, and acetylation in rice under starvation and submergence
    Year 2018
    Journal GENOME BIOLOGY
    Authors Lu Yue, et al.
    Applications Unspecified
    Reactivity
  • Protein Acylation is a General Regulatory Mechanism in Biosynthetic Pathway of Acyl-CoA-Derived Natural Products
    Year 2018
    Journal Cell Chemical Biology
    Authors Jun-Yu Xu, et al.
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  • LC–MS/MS-based quantitative study of the acyl group- and site-selectivity of human sirtuins to acylated nucleosomes
    Year 2018
    Journal Scientific Reports
    Authors Tanabe Kana, et al.
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  • A Class of Reactive Acyl-CoA Species Reveals the Non-enzymatic Origins of Protein Acylation
    Year 2017
    Journal Cell Metabolism
    Authors Gregory R. Wagner, et al.
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  • Characterization of histone acylations links chromatin modifications with metabolism
    Year 2017
    Journal Nature Communications
    Authors Simithy Johayra, et al.
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  • Lysine Malonylation Is Elevated in Type 2 Diabetic Mouse Models and Enriched in Metabolic Associated Proteins *
    Year 2015
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yipeng Du, et al.
    Applications Unspecified
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  • Aberrant protein acylation is a common observation in inborn errors of acyl-CoA metabolism
    Year 2014
    Journal JOURNAL OF INHERITED METABOLIC DISEASE
    Authors Pougovkina, et al.
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