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Recombinant Rabbit Monoclonal
Anti-Carboxyethyllysine Rabbit mAb
Catalog Number: PTM-1701RM
$ 350

Clone Number: 12H4L5

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB

Reactivity: All

Synonyms: Kcel

Product Size
100μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kcel
UniProt ID

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Immunogen Carboxyethylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Carboxyethyllysine Rabbit mAb detects proteins post-translationally modified by carboxyethylation on lysine residues. This pan antibody recognizes carboxyethylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

N epsilon-(carboxyethyl)lysine (CEL), one of the advanced glycation end products (AGEs), is a stable, nonenzymatic chemical modifications of protein lysine residues arising from glycation and oxidation. Kce can alter the structure and fuction of proteins, affecting their activity, stability, and interactions. This modification is closely related to the metabolic state of cells and may play an important role in metabolic stress and disease conditions. Kce has been reported to be associated with various pathological processes, including diabetic complications, atherosclerosis, and neurodegenerative diseases. Notably, thelevels of Kce are significantly elevated in diabetic complications and cardiovascular diseases.

Cellular location

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Images
Dot Blot

Peptide amount: 4 ng, 20 ng, 100ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided below.
Lane 1: Carboxyethyllysine peptide library. Dot 2: Glutaryllysine peptide library.
Dot 3: Succinyllysine peptide library. Dot 4: Malonyllysine peptide library.
Dot 5: Crotonyllysinepeptide library. Dot 6: Butyryllysine peptide library.
Dot 7: Propionyllysine peptide library. Dot 8: Acetyllysine peptide library.
Dot 9: L-lactyllysine peptide library. Dot 10: Unmodified peptide library.

Peptide amount: 10 ng, 20 ng, 50 ng, 100 ng, 500 ng
Blocking buffer: 3% BSA/TBST
Primary Ab dilution: 1:1500
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 15 seconds
The list of peptides used in the experiment is provided below.
Kce: Carboxyethyllysine peptide library. KD-la: D-lactyllysine peptide library.
KL-la: L-lactyllysine peptide library. Kac: Acetyllysine peptide library.
K: Unmodified peptide library.

Peptide amount: 10 ng, 20 ng, 50 ng, 100 ng
Blocking buffer: 3% BSA/TBST
Primary Ab dilution: 1:1500
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 15 seconds
The peptides used in the experiment are synthetic, sequence-specific histone peptides with the following modifications:
KL-la: L-lactyllysine peptide library. KD-la: D-lactyllysine peptide library.
Kce: Carboxyethyllysine peptide library.
WB

Samples: BSA, Acetylated BSA, L-lactylated BSA, D-lactylated BSA, Carboxyethylated BSA
Blocking buffer: 3% BSA/TBST
Primary Ab dilution: 1:1500
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 15 seconds

Predicted band size: 64 kDa

Observed band size: 64 kDa

Lysates: (-): HepG2 cells; (+): HepG2 cells + methylglyoxal (2 mM, 24 hours) + trichostatin A (1 μM, 5 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: (-): Rat brain; (+): Rat brain ground and exposed to UV (6 mJ/cm2, 2 hours), followed by protein extraction
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: (-): Mouse liver; (+): Mouse liver ground and exposed to UV (6 mJ/cm2, 2 hours), followed by protein extraction
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: 1. HeLa; 2. SH-SY5Y; 3. BRL; 4. Neuro2a
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: 1. Rat liver; 2. Mouse brain
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Research Use

For research use only, not for use in diagnostic procedures.

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