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Recombinant Rabbit Monoclonal
Anti-CD1a Rabbit mAb
Catalog Number: PTM-5015
$ 290

Clone Number: JRMR-51

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： IHC-P FC mIHC-P

Reactivity: Human

Synonyms: hTa1 thymocyte antigen

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms hTa1 thymocyte antigen
UniProt ID

P06126

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
IHC-P 1:500 - 1:2000 Human
mIHC-P 1:200 - 1:1000 Human
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Cellular location

Cytoplasm

Images
IHC-P

Tissue: Human esophagus
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:2000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5015

Tissue: Human skin
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:2000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5015

Tissue: Human squamous cervical carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:2000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5015

Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:2000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5015
mIHC-P

Tissue: Human esophagus
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0.
Heat mediated antigen retrieval (Tris/EDTA buffer, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.
Primary Ab dilution: PTM-5015 (1:1000), PTM_x0002_5012 (1:1000), PTM-5032 (1:100)
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Nuclear counter stain: DAPI
Description: The red color represents the positive signal observed with CD1a (PTM-5015, TSA-570), The green color represents the positive signal observed with CK4 (PTM-5012, TSA-520), color yellow is the positive signal for Ki67 (PTM_x0002_5032, TSA-650). The section was incubated in three rounds of staining; in the order of PTM5012, PTM-5015, PTM-5032; each using a separate fluorescent tyramide signal amplification system.
FC

Cell line: MOLT4
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:50
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-5015

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 280
    Recombinant
    Anti-CD1a Mouse mAb
    Catalog #
    PTM-5201
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
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