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Recombinant Rabbit Monoclonal
Anti-CD42b Rabbit mAb
Catalog Number: PTM-7037
$ 300

Clone Number: JRMR-10921-28

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P

Reactivity: Human

Synonyms: GP1BA, GP-Ib alpha, GPIbA

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms GP1BA, GP-Ib alpha, GPIbA
UniProt ID

P07359

Immunogen
MW (kDa) 130
Specificity Anti-CD42b Rabbit mAb recognizes endogeneous level of CD42b protein.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:5000 Human
IHC-P 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

CD42b, also named as GP1BA, is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

Cellular location

Membrane

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate)
Lysate: 1: Human platelet
Protein loading amount: 20 μg
Exposure time: 60 seconds
Predicted band size: 72 kDa
Observed band size: 130 kDa

IHC-P

Tissue: Human spleen
Section type: Formalin-fixed & paraffinembedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for PTM-7037

Tissue: Human liver (Negative tissue)
Section type: Formalin-fixed & paraffinembedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: No staining on PTM-7037

Research Use

For research use only, not for use in diagnostic procedures.

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