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Anti-Citrulline-Histone H2A (Arg3) Rabbit pAb
Catalog Number: PTM-1305
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: H2AR3cit

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H2AR3cit
UniProt ID

P04908

Immunogen Citrullinated human histone H2A (Arg3) peptide
MW (kDa) 14
Specificity Anti-Citrulline-Histone H2A (Arg3) Rabbit pAb detects histone H2A only when it is citrullinated at Arg3.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Protein citrullination is an emerging PTM that results from the conversion of peptidyl arginine to peptidyl citrulline. Due to the exchange of an imine for a carbonyl group, this reaction is referred to as deimination. This PTM is catalyzed by the calcium-regulated peptidylarginine deiminases (PAD). Dysregulated PAD expression and aberrant protein citrullination have been implicated in numerous human diseases such as osteosarcoma, colorectal cancer, esophagus cancer and breast cancer.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided in the table below.
WB

Lysate: (-): HL-60; (+): HL-60 + calcium ionophore A (4 μM, 15 minutes)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 14 kDa
Observed band size: 14 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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