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Recombinant Rabbit Monoclonal
Anti-Cleaved PARP1 p25 Rabbit mAb
Catalog Number: PTM-5272
$ 320

Clone Number: JRMR-243

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF

Reactivity: Human, Mouse

Synonyms: ADPRT1, PPOL

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms ADPRT1, PPOL
UniProt ID

P09874

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse
IHC-P 1:200 - 1:1000 Human
ICC/IF 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Lysate: HeLa, Raw264.7, Jurkat, 3T3
Protein loading quantity: 20 μg
Exposure time: 60 S
Predicted band size: 25 kDa
Observed band size: 25 kDa
IHC-P

Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-5272

Tissue: Human ovarian carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-5272
ICC/IF

Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:100
Primary incubation condition: 4°C overnight
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed withPTM-5272

Research Use

For research use only, not for use in diagnostic procedures.

References (1)
  • (2E)-1-(2,4,6-Trimethoxyphenyl)-3-(4-chlorophenyl)prop-2-en-1-one, a Chalcone Derivative, Promotes Apoptosis by Suppressing RAS-ERK and AKT/FOXO3a Pathways in Hepatocellular Carcinoma Cells
    Year 2023
    Journal CHEMISTRY & BIODIVERSITY
    Authors Meigui Zhang, et al.
    Applications Unspecified
    Reactivity
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