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Anti-COUP-TFII Rabbit mAb
Catalog Number: PTM-7113
$ 280

Clone Number:

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P ChIP

Reactivity:

Synonyms:

Product Size

Shipping:

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype
Conjugate
Synonyms
UniProt ID

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
Properties
Purity
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Cellular location

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: MCF-7, 2: K562, 3: PC-12
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 46 kDa
Observed MW: 46 kDa

IHC-P

Tissue: Human testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7113

Tissue: Human breast carcinoma
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7113

Tissue: Mouse testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7113

Tissue: Rat testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7113

Research Use

For research use only, not for use in diagnostic procedures.