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Recombinant
Anti-COX IV Mouse mAb
Catalog Number: PTM-6528
$ 125

Clone Number: 10664

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB FC

Reactivity: Human

Synonyms: COX4I1, COX4, COX4-1, COXIV, COX IV-1, COXIV-1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms COX4I1, COX4, COX4-1, COXIV, COX IV-1, COXIV-1
UniProt ID

P13073

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
FC 1:50 - 1:200 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

COXIV, also named as COX4, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV is a marker for mitochondria, which has two isoforms (isoform 1 and 2). Isoform 1 is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COXIV is commonly used as a loading control.

Cellular location

Mitochondrion

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)
Lysate: 1: HeLa, 2: A549, 3: HepG2, 4: Jurkat
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 20 kDa
Observed band size: 15 kDa

FC

Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:200
Secondary Ab: Goat Anti-Mouse IgG
Unlabeled control: Cells without incubationwith primary and secondary antibodies(Black line).
Isotype control: Mouse monoclonal IgG1 (Blueline).
Description: Line red is the positive signal for PTM-6528

Research Use

For research use only, not for use in diagnostic procedures.

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