中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-COX IV Rabbit mAb (HRP Conjugate)
Catalog Number: PTM-6675
$ 395

Clone Number: JRMR-10858-114

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: COX4

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate HRP
Synonyms COX4
UniProt ID

P13073

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:5000 Human, Mouse, Rat
IHC-P 1:100 - 1:500 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

COX IV is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX IV is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody targets COX4I1 and was conjugated with HRP, thus secondary antibody incubation step could be omitted.

Cellular location

Mitochondrion

Images
IHC-P

Tissue: Human kidney
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6675

Tissue: Human liver
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6675
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Lysate: 1: HepG2, 2: HeLa, 3: Jurkat
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 20 kDa
Observed band size: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Lysate: 1: Mouse heart, 2: Rat heart
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 20 kDa
Observed band size: 15 kDa

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
Compatible Secondaries Isotype Control Writers/Readers/Erasers Related Products
  • View More Images（6）
    $ 130
    Recombinant Rabbit Monoclonal
    Anti-COX IV Rabbit mAb
    Catalog #
    PTM-6421
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（3）
    $ 125
    Recombinant
    Anti-COX IV Mouse mAb
    Catalog #
    PTM-5937
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 125
    Recombinant
    Anti-COX IV Mouse mAb
    Catalog #
    PTM-5908
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 125
    Recombinant
    Anti-COX IV Mouse mAb
    Catalog #
    PTM-6528
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.