Applications：
WB
IHC-P
ICC/IF
FC
IAP
IP
ChIP
mIHC-P
Clone Number: /
Host: Mouse Clonality: Monoclonal
Applications： WB
Reactivity: Human, Mouse, Rat, Monkey
Synonyms: H2BK11cr
Shipping: Ambient temperature
Order online or send purchase order to info@ptmbio.com
| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | H2BK11cr |
| UniProt ID | |
| Immunogen | Crotonylated human histone H2B (Lys11) peptide |
| MW (kDa) | 14 |
| Specificity |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:2000 | Human, Mouse, Rat, Monkey |
| Purity | Protein G and immunogen affinity purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H2B at Lys11 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.
Cellular location
Nucleus
| Dot Blot | |
|---|---|
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Blocking buffer: 5% NFDM/TBST |
| WB | |
|---|---|
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Blocking buffer: 5% NFDM/TBST |
Research Use
For research use only, not for use in diagnostic procedures.
