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Recombinant Rabbit Monoclonal
Anti-Crotonyl-histone H3 (Lys18/23) Rabbit mAb
Catalog Number: PTM-544RM
$ 435

Clone Number: 10H3L2

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF IP

Reactivity: Human, Mouse, Rat

Synonyms: H3K18/K23cr

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K18/K23cr
UniProt ID

P68431

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1: 500-1: 2000 Human, Rat, Mouse
ICC/IF 1: 50 Human
IP 1: 50-1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys18/Lys23 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 1ng, 4 ng, 16 ng, 64 ng
Exposure time: 30 seconds
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa+crotonic(100ng/ml, 18hs)
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, 3T3, BRL
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
ICC/IF

Cell line: (A) HeLa, (B)HeLa+crotonic acid (100 ng/ml, 18hr)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-544RM
IP

IP of HeLa+crotonic acid (100 ng/ml, 18hr) cells extracts
IP Ab incubation condition: PTM-544RM, 4°C overnight, 1:100 dilution
WB Primary Ab incubation condition: PTM-544RM, room temperature 2h, 1:2000
dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
1: 5% Input
2: IP with PTM-544RM
3: IP with Rabbit monoclonal IgG Isotype
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 60 s

Research Use

For research use only, not for use in diagnostic procedures.

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