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Recombinant Rabbit Monoclonal
Anti-Crotonyl-Histone H3 (Lys18) Rabbit mAb
Catalog Number: PTM-517RM
$ 420

Clone Number: B1169/344-H1L1

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: H3K18cr

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K18cr
UniProt ID

P68431

Immunogen Crotonylated human histone H3 (Lys18) peptide
MW (kDa) 15
Specificity Anti-Crotonyl-histone H3 (Lys18) Rabbit mAb detects histone H3 only when it is crotonylated at Lys18.
Product Usage Information
Applications Dilution Recommended Species
WB 1:2000-1:10000 Human, Mouse, Rat
ICC/IF 1:200-1:1000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Lysine crotonylation is a newly-identified histone post-translational modification that has come to light through integrated proteomic approaches and elaborate biochemistry analyses. Lysine crotonylation has been found to be evolutionarily conserved across multiple species, spanning both prokaryotes and eukaryotes, and occurs in a wide range of proteins, incuding histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1ng, 4 ng, 16 ng, 64 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 10 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K18 crotonyl. Dot 2: H3K14 acetyl.
Dot 3: H3K18 acetyl. Dot 4: H3K23 acetyl.
Dot 5: H3K14 butyryl. Dot 6: H3K18 butyryl.
Dot 7: H3K23 butyryl. Dot 8: H3K14 crotonyl.
Dot 9: H3K23 crotonyl. Dot 10: H3K14 propionyl.
Dot 11: H3K18 propionyl. Dot 12: H3K23 propionyl.
Dot 13: H3K18 unmodified.
WB

Lysates: (-): HeLa cells; (+): HeLa cells treated with 10 mM crotonic acid for 15 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Lysates: (-): Neuro-2a cells; (+): Neuro-2a cells treated with 10 mM crotonic acid for 15 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Lysates: (-): C6 cells; (+): C6 cells treated with 10 mM crotonic acid for 15 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
ICC/IF

Samples: (A) 293T cells; (B) HeLa 293T treated with 10 mM crotonic acid for 15 hours
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-517RM.

Research Use

For research use only, not for use in diagnostic procedures.

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