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Anti-Crotonyl-Histone H3 (Lys18) Rabbit pAb
Catalog Number: PTM-517
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: H3K18cr

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K18cr
UniProt ID

P68431

Immunogen Crotonylated human histone H3 (Lys18) peptide
MW (kDa) 15
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
IHC-P 1:50 - 1:100 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 30 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa+Sodium crotonylate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 17 kDa
Observed band size: 17 kDa
IHC-P

Tissue: Human cerebrum
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-517

Tissue: Human colon carcinoma
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-517

Tissue: Human kidney
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-517

Tissue: Human spleen
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-517

Tissue: Human thyroid carcinoma
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-517

Research Use

For research use only, not for use in diagnostic procedures.

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References (12)
  • Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease
    Year 2022
    Journal JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
    Authors Lin Dang, et al.
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  • Sodium butyrate mediates histone crotonylation and alleviated neonatal rats hypoxic-ischemic brain injury through gut-brain axis.
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    Journal Frontiers in Microbiology
    Authors Xuejia He, et al.
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  • Sorafenib Attenuates Fibrotic Hepatic Injury Through Mediating Lysine Crotonylation
    Year 2022
    Journal Drug Design Development and Therapy
    Authors Xiao-Feng Chen, et al.
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  • Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis
    Year 2021
    Journal Nature Chemical Biology
    Authors Song Xiaoyu, et al.
    Applications Unspecified
    Reactivity
  • The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum
    Year 2021
    Journal eLife
    Authors Ning Zhang, et al.
    Applications Unspecified
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  • Gcn5 and Esa1 function as histone crotonyltransferases to regulate crotonylation-dependent transcription
    Year 2019
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Leonie Kollenstart, et al.
    Applications Unspecified
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  • Microbiota derived short chain fatty acids promote histone crotonylation in the colon through histone deacetylases
    Year 2018
    Journal Nature Communications
    Authors Fellows Rachel, et al.
    Applications Unspecified
    Reactivity
  • Histone deacetylase (HDAC) 1 and 2 complexes regulate both histone acetylation and crotonylation in vivo
    Year 2018
    Journal Scientific Reports
    Authors Kelly R. D. W., et al.
    Applications Unspecified
    Reactivity
  • In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones
    Year 2018
    Journal Bio-protocol
    Authors Rachel Fellows, et al.
    Applications Unspecified
    Reactivity
  • Class I histone deacetylases are major histone decrotonylases: evidence for critical and broad function of histone crotonylation in transcription
    Year 2017
    Journal CELL RESEARCH
    Authors Wei Wei, et al.
    Applications Unspecified
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  • Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis
    Year 2017
    Journal MOLECULAR CELL
    Authors Shumeng Liu, et al.
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  • MOF as an evolutionarily conserved histone crotonyltransferase and transcriptional activation by histone acetyltransferase-deficient and crotonyltransferase-competent CBP/p300
    Year 2017
    Journal Cell Discovery
    Authors Liu Xiaoguang, et al.
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