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Anti-Crotonyllysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-503
$ 2,110

Clone Number: /

Host: / Clonality: /

Applications: IAP

Reactivity: All

Synonyms: Kcr

Product Size
500 μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms Kcr
UniProt ID

/

Immunogen Crotonylated lysine peptides
MW (kDa) Multiple
Specificity With a mixture of high-quality rabbit- and mouse-derived anti-crotonyllysine antibodies cross-linked, this Anti-Crotonyllysine Antibody Conjugated Agarose Beads present extraordinary capability to specifically capture peptides/proteins bearing crotonyllysine residues but do not cross-react with the peptides/proteins bearing structurally similar acetyllysine residues. This product has been well utilized to affinity purify peptides for global proteomic screening of lysine crotonylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Lysine crotonylation is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine crotonylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by effecting chromatin structure and epigenetic pathways.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-Crotonyllysine Rabbit pAb (PTM-501) on different acylated proteins.
Protein amount: 1 ng, 5 ng, 25 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of proteins used in the experiment is provided below.
Lane 1: Crotonylated BSA. Lane 2: Acetylated BSA. Lane 3: Unmodified BSA.

Research Use

For research use only, not for use in diagnostic procedures.

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References (45)
  • The faucet knob effect of DptE crotonylation on the initial flow of daptomycin biosynthesis
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    Authors Wen-Li Gao, et al.
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    Reactivity
  • Deep Crotonylproteomics Analysis of High-Altitude Exposure Hearts in Mice
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    Authors Tian Yue, et al.
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  • Potential functions and mechanisms of lysine crotonylation modification (Kcr) in tumorigenesis and lymphatic metastasis of papillary thyroid cancer (PTC)
    Year 2024
    Journal Journal of Translational Medicine
    Authors Li Zhaokun, et al.
    Applications Unspecified
    Reactivity
  • Hypoxia-induced downregulation of PGK1 crotonylation promotes tumorigenesis by coordinating glycolysis and the TCA cycle
    Year 2024
    Journal Nature Communications
    Authors Guo Zihao, et al.
    Applications Unspecified
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  • Global proteomic analyses of lysine acetylation, malonylation, succinylation, and crotonylation in human sperm reveal their involvement in male fertility
    Year 2024
    Journal Journal of Proteomics
    Authors Yan Tian, et al.
    Applications Unspecified
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  • YjgM is a crotonyltransferase critical for polymyxin resistance of Escherichia coli
    Year 2024
    Journal Cell Reports
    Authors Jianpeng Zhuang, et al.
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  • Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate
    Year 2023
    Journal Science Advances
    Authors Ning Zhang, et al.
    Applications Unspecified
    Reactivity
  • Systematic analysis of lysine crotonylation in human macrophages responding to MRSA infection
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    Authors Zhang Hao, et al.
    Applications Unspecified
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  • Qualitative lysine crotonylation and 2-hydroxyisobutyrylation analysis in the ovarian tissue proteome of piglets
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    Authors Yang Diqi, et al.
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  • Large-scale lysine crotonylation analysis reveals the role of TRAF6-Ecsit complex in endoplasmic reticulum stress in mud crab (Scylla paramamosain)
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  • Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer
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  • Modulation of cellular metabolism by protein crotonylation regulates pancreatic cancer progression
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  • SEPT2 crotonylation promotes metastasis and recurrence in hepatocellular carcinoma and is associated with poor survival
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  • Adenosine-rich extract of Ganoderma lucidum: A safe and effective lipid-lowering substance
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  • Regulation of Cellular Metabolism by Protein Crotonylation Coordinates Pancreatic Cancer Progression
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  • Proteome-Wide Identification and Functional Analysis of Lysine Crotonylation in Trichophyton rubrum Conidial and Mycelial Stages
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    Authors Xingye Xu, et al.
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  • Lysine Acylation Modification Landscape of Brucella abortus Proteome and its Virulent Proteins
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    Applications Unspecified
    Reactivity
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    Applications Unspecified
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    Authors Huajing Yu, et al.
    Applications Unspecified
    Reactivity
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    Applications Unspecified
    Reactivity
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    Year 2018
    Journal GENOME BIOLOGY
    Authors Lu Yue, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive analysis of lysine crotonylation in proteome of maintenance hemodialysis patients
    Year 2018
    Journal MEDICINE
    Authors Wenbiao Chen, et al.
    Applications Unspecified
    Reactivity
  • Global Involvement of Lysine Crotonylation in Protein Modification and Transcription Regulation in Rice
    Year 2018
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Shuai Liu, et al.
    Applications Unspecified
    Reactivity
  • A qualitative proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.)
    Year 2018
    Journal Scientific Reports
    Authors Liu Kaidong, et al.
    Applications Unspecified
    Reactivity
  • First profiling of lysine crotonylation of myofilament proteins and ribosomal proteins in zebrafish embryos
    Year 2018
    Journal Scientific Reports
    Authors Kwon Oh Kwang, et al.
    Applications Unspecified
    Reactivity
  • Ultradeep Lysine Crotonylome Reveals the Crotonylation Enhancement on Both Histones and Nonhistone Proteins by SAHA Treatment
    Year 2017
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    Authors Quan Wu, et al.
    Applications Unspecified
    Reactivity
  • First comprehensive proteome analysis of lysine crotonylation in seedling leaves of Nicotiana tabacum
    Year 2017
    Journal Scientific Reports
    Authors Sun Hangjun, et al.
    Applications Unspecified
    Reactivity
  • SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability
    Year 2016
    Journal Nature Communications
    Authors Li Lei, et al.
    Applications Unspecified
    Reactivity