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Anti-Crotonyllysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-503
$ 1,920

Clone Number: /

Host: / Clonality: /

Applications： IAP

Reactivity: All

Synonyms: Kcr

Product Size
500 μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms Kcr
UniProt ID

/

Immunogen Crotonylated lysine peptides
MW (kDa) Multiple
Specificity With a mixture of high-quality rabbit- and mouse-derived anti-crotonyllysine antibodies cross-linked, this Anti-Crotonyllysine Antibody Conjugated Agarose Beads present extraordinary capability to specifically capture peptides/proteins bearing crotonyllysine residues but do not cross-react with the peptides/proteins bearing structurally similar acetyllysine residues. This product has been well utilized to affinity purify peptides for global proteomic screening of lysine crotonylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Lysine crotonylation is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine crotonylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by effecting chromatin structure and epigenetic pathways.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-Crotonyllysine Rabbit pAb (PTM-501) on different acylated proteins.
Protein amount: 1 ng, 5 ng, 25 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of proteins used in the experiment is provided below.
Lane 1: Crotonylated BSA. Lane 2: Acetylated BSA. Lane 3: Unmodified BSA.

Research Use

For research use only, not for use in diagnostic procedures.

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