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Recombinant Rabbit Monoclonal
Anti-Crotonyllysine Mouse mAb
Catalog Number: PTM-502
$ 420

Clone Number: 4D5

Host: Mouse Clonality: Monoclonal

Applications: WB

Reactivity: All

Synonyms: Kcr

Product Size
100μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kcr
UniProt ID

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Immunogen Crotonylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Crotonyllysine Mouse mAb detects proteins with crotonylated lysine residues. This pan antibody recognizes crotonylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Lysine crotonylation is a newly-identified histone post-translational modification that has come to light through integrated proteomic approaches and elaborate biochemistry analyses. Lysine crotonylation has been found to be evolutionarily conserved across multiple species, spanning both prokaryotes and eukaryotes, and occurs in a wide range of proteins, incuding histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by exerting its effects on chromatin structure and epigenetic pathways.

Cellular location

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Images
Dot Blot

Peptide/protein amount: 100 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides and proteins used in the experiment is provided below.
Dot A1: crotonylated peptide library.

Dot A2-A7: histone site specific crotonyllysine peptides.
Dot A8: acetylated peptide library.

Dot A9: unmodified histone site specific peptides.
Dot B1: crotonylated BSA.

Dot B2-B6: histone site specific crotonyllysine peptides.
Dot B7: unmodified peptide library.

Dot B8: propionylated peptide library.
Dot B9: unmodified histone site specific peptides.

Dot C1-C6: histone site specific crotonyllysine peptides.
Dot C7: unmodified BSA.

Dot C8: butyrylated peptide library.
Dot C9: unmodified histone site specific peptides.

WB

Lysate: HeLa cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Research Use

For research use only, not for use in diagnostic procedures.

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References (43)
  • Linking chromatin acylation mark-defined proteome and genome in living cells
    Year 2023
    Journal CELL
    Authors Fangfei Qin, et al.
    Applications Unspecified
    Reactivity
  • Core-predominant gut fungus Kazachstania slooffiae promotes intestinal epithelial glycolysis via lysine desuccinylation in pigs
    Year 2023
    Journal Microbiome
    Authors Hu Jun, et al.
    Applications Unspecified
    Reactivity
  • Lysine 2-Hydroxyisobutyrylation- and Succinylation-Based Pathways Act Inside Chloroplasts to Modulate Plant Photosynthesis and Immunity
    Year 2023
    Journal Advanced Science
    Authors Bin Chen, et al.
    Applications Unspecified
    Reactivity
  • Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate
    Year 2023
    Journal Science Advances
    Authors Ning Zhang, et al.
    Applications Unspecified
    Reactivity
  • Chloroplast crotonylome analysis of wheat seedling leaves reveals the roles of crotonylated proteins involved in salt stress response
    Year 2023
    Journal JOURNAL OF EXPERIMENTAL BOTANY
    Authors Zhu Dong, et al.
    Applications Unspecified
    Reactivity
  • Acetyl-Proteomic Profiling of Sorghum bicolor Seedlings after Chitin Treatment Reveals the Involvement of Acetylated Chlorophyll a/b Binding Proteins in the Innate Immune Response
    Year 2023
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Junmei Jiang, et al.
    Applications Unspecified
    Reactivity
  • Systematic analysis of lysine crotonylation in human macrophages responding to MRSA infection
    Year 2023
    Journal Frontiers in Cellular and Infection Microbiology
    Authors Zhang Hao, et al.
    Applications Unspecified
    Reactivity
  • Qualitative lysine crotonylation and 2-hydroxyisobutyrylation analysis in the ovarian tissue proteome of piglets
    Year 2023
    Journal Frontiers in Cell and Developmental Biology
    Authors Diqi Yang, et al.
    Applications Unspecified
    Reactivity
  • HDAC1 inhibits beige adipocyte-mediated thermogenesis through histone crotonylation of Pgc1a/Ucp1
    Year 2023
    Journal CELLULAR SIGNALLING
    Authors Dingyuan Tian, et al.
    Applications Unspecified
    Reactivity
  • Development of Lysine Crotonyl-mimic Probe to Covalently Identify H3K27Cr Interacting Proteins
    Year 2023
    Journal CHEMISTRY-A EUROPEAN JOURNAL
    Authors Xiaochun Guo, et al.
    Applications Unspecified
    Reactivity
  • Large-scale lysine crotonylation analysis reveals the role of TRAF6-Ecsit complex in endoplasmic reticulum stress in mud crab (Scylla paramamosain)
    Year 2023
    Journal DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
    Authors Yongsheng Zhang, et al.
    Applications Unspecified
    Reactivity
  • Remodeling of the 3D chromatin architecture in the marine microalga Nannochloropsis oceanica during lipid accumulation
    Year 2023
    Journal Biotechnology for Biofuels and Bioproducts
    Authors Yan Tongtong, et al.
    Applications Unspecified
    Reactivity
  • Dynamic switching of crotonylation to ubiquitination of H2A at lysine 119 attenuates transcription–replication conflicts caused by replication stress
    Year 2022
    Journal NUCLEIC ACIDS RESEARCH
    Authors Hao Shuailin, et al.
    Applications Unspecified
    Reactivity
  • KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity
    Year 2022
    Journal Autophagy
    Authors Guokai Yan, et al.
    Applications Unspecified
    Reactivity
  • Persistent high glucose induced EPB41L4A-AS1 inhibits glucose uptake via GCN5 mediating crotonylation and acetylation of histones and non-histones
    Year 2022
    Journal Clinical and Translational Medicine
    Authors Weijie Liao, et al.
    Applications Unspecified
    Reactivity
  • Regulation of Cellular Metabolism by Protein Crotonylation Coordinates Pancreatic Cancer Progression
    Year 2022
    Journal Cell Reports
    Authors Zheng Yan, et al.
    Applications Unspecified
    Reactivity
  • Lysine crotonylation regulates leucine-deprivation-induced autophagy by a 14-3-3ε-PPM1B axis
    Year 2022
    Journal Cell Reports
    Authors Zilong Zheng, et al.
    Applications Unspecified
    Reactivity
  • Global crotonylome reveals hypoxia-mediated lamin A crotonylation regulated by HDAC6 in liver cancer
    Year 2022
    Journal Cell Death & Disease
    Authors Zhang Dan, et al.
    Applications Unspecified
    Reactivity
  • Acox2 is a regulator of lysine crotonylation that mediates hepatic metabolic homeostasis in mice
    Year 2022
    Journal Cell Death & Disease
    Authors Zhang Yuan, et al.
    Applications Unspecified
    Reactivity
  • Protein Lactylation and Metabolic Regulation of the Zoonotic Parasite Toxoplasma gondii
    Year 2022
    Journal GENOMICS PROTEOMICS & BIOINFORMATICS
    Authors Deqi Yin, et al.
    Applications Unspecified
    Reactivity
  • Adenosine-rich extract of Ganoderma lucidum: a safe and effective lipid-lowering substance via regulating expression and modulation of PPAR signaling pathway
    Year 2022
    Journal iScience
    Authors He Li, et al.
    Applications Unspecified
    Reactivity
  • DEAD-Box RNA Helicase DDX47 Maintains Midgut Homeostasis in Locusta migratoria
    Year 2022
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Jun-Xiu Wang, et al.
    Applications Unspecified
    Reactivity
  • Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense.
    Year 2022
    Journal Frontiers in Plant Science
    Authors Wu J, et al.
    Applications Unspecified
    Reactivity
  • Cold Atmospheric Plasma Conveys Selectivity Against Hepatocellular Carcinoma Cells via Triggering EGFR(Tyr1068)-Mediated Autophagy
    Year 2022
    Journal Frontiers in Oncology
    Authors Danjun Wang, et al.
    Applications Unspecified
    Reactivity
  • Integrated insights into the mechanisms underlying sepsis-induced myocardial depression using a quantitative global proteomic analysis
    Year 2022
    Journal Journal of Proteomics
    Authors Ni Yang, et al.
    Applications Unspecified
    Reactivity
  • The single-cell epigenomic and transcriptional landscape of immunity to influenza vaccination
    Year 2021
    Journal CELL
    Authors Florian Wimmers, et al.
    Applications Unspecified
    Reactivity
  • Histone crotonylation promotes mesoendodermal commitment of human embryonic stem cells
    Year 2021
    Journal Cell Stem Cell
    Authors Yi Fang, et al.
    Applications Unspecified
    Reactivity
  • Toxoplasma gondii Infection Inhibits Histone Crotonylation to Regulate Immune Response of Porcine Alveolar Macrophages
    Year 2021
    Journal Frontiers in Immunology
    Authors Jing Yang, et al.
    Applications Unspecified
    Reactivity
  • SIRT7-Induced PHF5A Decrotonylation Regulates Aging Progress Through Alternative Splicing-Mediated Downregulation of CDK2
    Year 2021
    Journal Frontiers in Cell and Developmental Biology
    Authors Ai Qing Yu, et al.
    Applications Unspecified
    Reactivity
  • Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in Candida albicans | mSystems
    Year 2021
    Journal mSystems
    Authors Xiaowei Zhou, et al.
    Applications Unspecified
    Reactivity
  • Deficient histone H3 propionylation by BRPF1-KAT6 complexes in neurodevelopmental disorders and cancer
    Year 2020
    Journal Science Advances
    Authors Kezhi Yan, et al.
    Applications Unspecified
    Reactivity
  • Global Proteomic Analysis of Lysine Crotonylation in the Plant Pathogen Botrytis cinerea
    Year 2020
    Journal Frontiers in Microbiology
    Authors Zhang Ning, et al.
    Applications Unspecified
    Reactivity
  • Crotonylation of key metabolic enzymes regulates carbon catabolite repression in Streptomyces roseosporus
    Year 2020
    Journal Communications Biology
    Authors Sun Chen-Fan, et al.
    Applications Unspecified
    Reactivity
  • Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis
    Year 2020
    Journal Journal of Proteomics
    Authors Chenchang Bao, et al.
    Applications Unspecified
    Reactivity
  • Functional Oocytes Derived from Granulosa Cells
    Year 2019
    Journal Cell Reports
    Authors Chenglei Tian, et al.
    Applications Unspecified
    Reactivity
  • Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy
    Year 2019
    Journal JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
    Authors Hadi R. Ali, et al.
    Applications Unspecified
    Reactivity
  • Global Lysine Crotonylation and 2-Hydroxyisobutyrylation in Phenotypically Different Toxoplasma gondii Parasites *
    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Deqi Yin, et al.
    Applications Unspecified
    Reactivity
  • Characterization of the Sperm Proteome and Reproductive Outcomes with in Vitro, Fertilization after a Reduction in Male Ejaculatory Abstinence Period *
    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Zi-Qi Shen, et al.
    Applications Unspecified
    Reactivity
  • Ammonium triggered the response mechanism of lysine crotonylome in tea plants
    Year 2019
    Journal BMC GENOMICS
    Authors Sun Jianhao, et al.
    Applications Unspecified
    Reactivity
  • Dose-response regulation system for improving renal injury in diabetic nephropathy by buckwheat hull flavonoids
    Year 2019
    Journal Journal of Functional Foods
    Authors Yufang Tang, et al.
    Applications Unspecified
    Reactivity
  • Elevated lysine crotonylation and succinylation in the brains of BTBR mice
    Year 2019
    Journal INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
    Authors Min Wang, et al.
    Applications Unspecified
    Reactivity
  • Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging
    Year 2018
    Journal CELL
    Authors Peggie Cheung, et al.
    Applications Unspecified
    Reactivity
  • Lysine Malonylation Is Elevated in Type 2 Diabetic Mouse Models and Enriched in Metabolic Associated Proteins *
    Year 2015
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity