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Anti-Crotonyllysine Rabbit pAb
Catalog Number: PTM-501
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyconal

Applications: WB

Reactivity: All

Synonyms: Kcr

Product Size
100μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kcr
UniProt ID

/

Immunogen Crotonylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Crotonyllysine Rabbit pAb detects proteins with crotonylated lysine residues. This pan antibody recognizes crotonylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Lysine crotonylation is a newly-identified histone post-translational modification that has come to light through integrated proteomic approaches and elaborate biochemistry analyses. Lysine crotonylation has been found to be evolutionarily conserved across multiple species, spanning both prokaryotes and eukaryotes, and occurs in a wide range of proteins, incuding histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by exerting its effects on chromatin structure and epigenetic pathways.

Cellular location

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Images
Dot Blot

Protein amount: 1 ng, 5 ng, 25 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of proteins used in the experiment is provided below.
Lane 1: Crotonylated BSA. Lane 2: Acetylated BSA. Lane 3: BSA.

WB

Samples: Core histones from HeLa Cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab: Lane 1, PTM-501 + unmodified BSA. Lane 2, PTM-501 + crotonylated BSA
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Research Use

For research use only, not for use in diagnostic procedures.

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References (62)
  • Fasting-Mimicking Diet Prevents Pancreatic Carcinogenesis via Gut Microbiota and Metabolites
    Year 2024
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Huaxin Li, et al.
    Applications Unspecified
    Reactivity
  • PTBP1 crotonylation promotes colorectal cancer progression through alternative splicing-mediated upregulation of the PKM2 gene
    Year 2024
    Journal Journal of Translational Medicine
    Authors Hou Jia-Yi, et al.
    Applications Unspecified
    Reactivity
  • Hypoxia-induced downregulation of PGK1 crotonylation promotes tumorigenesis by coordinating glycolysis and the TCA cycle
    Year 2024
    Journal Nature Communications
    Authors Guo Zihao, et al.
    Applications Unspecified
    Reactivity
  • Pyruvate dehydrogenase complex E1 subunit α crotonylation modulates cocaine-associated memory through hippocampal neuron activation
    Year 2024
    Journal Cell Reports
    Authors Hongchun Li, et al.
    Applications Unspecified
    Reactivity
  • Astrocyte-derived lactate aggravates brain injury of ischemic stroke in mice by promoting the formation of protein lactylation
    Year 2024
    Journal Theranostics
    Authors Xiao-Yi Xiong, et al.
    Applications Unspecified
    Reactivity
  • p53 deficiency mediates cisplatin resistance by upregulating RRM2 and crotonylation of RRM2K283 through the downregulation of SIRT7
    Year 2024
    Journal Frontiers in Molecular Biosciences
    Authors Sun Liangjie, et al.
    Applications Unspecified
    Reactivity
  • Sodium Crotonate Alleviates Diabetic Kidney Disease Partially Via the Histone Crotonylation Pathway
    Year 2024
    Journal INFLAMMATION
    Authors He Yanqiu, et al.
    Applications Unspecified
    Reactivity
  • SIRT1 maintains bone homeostasis by regulating osteoblast glycolysis through GOT1
    Year 2024
    Journal CELLULAR AND MOLECULAR LIFE SCIENCES
    Authors Jin Xinxin, et al.
    Applications Unspecified
    Reactivity
  • Lysine catabolism reprograms tumour immunity through histone crotonylation
    Year 2023
    Journal NATURE
    Authors Yuan Huairui, et al.
    Applications Unspecified
    Reactivity
  • Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner
    Year 2023
    Journal CELL RESEARCH
    Authors Liao Long, et al.
    Applications Unspecified
    Reactivity
  • Hypoxia Increases ATX Expression by Histone Crotonylation in a HIF-2α-Dependent Manner
    Year 2023
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Mengxia Qu, et al.
    Applications Unspecified
    Reactivity
  • The short-chain fatty acid crotonate reduces invasive growth and immune escape of Candida albicans by regulating hyphal gene expression
    Year 2023
    Journal mBio
    Authors Christopher McCrory, et al.
    Applications Unspecified
    Reactivity
  • Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer
    Year 2023
    Journal Epigenetics & Chromatin
    Authors Hou Jia-Yi, et al.
    Applications Unspecified
    Reactivity
  • Modulation of cellular metabolism by protein crotonylation regulates pancreatic cancer progression
    Year 2023
    Journal Cell Reports
    Authors Yan Zheng, et al.
    Applications Unspecified
    Reactivity
  • Hypoxia Increases ATX Expression by Histone Crotonylation in a HIF-2α-Dependent Manner
    Year 2023
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Mengxia Qu, et al.
    Applications Unspecified
    Reactivity
  • Crotonylation of GAPDH regulates human embryonic stem cell endodermal lineage differentiation and metabolic switch
    Year 2023
    Journal Stem Cell Research & Therapy
    Authors Zhang Jingran, et al.
    Applications Unspecified
    Reactivity
  • Sodium butyrate mediates histone crotonylation and alleviated neonatal rats hypoxic-ischemic brain injury through gut-brain axis.
    Year 2022
    Journal Frontiers in Microbiology
    Authors Xuejia He, et al.
    Applications Unspecified
    Reactivity
  • Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease
    Year 2022
    Journal JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
    Authors Dang Lin, et al.
    Applications Unspecified
    Reactivity
  • Global crotonylome reveals hypoxia-mediated lamin A crotonylation regulated by HDAC6 in liver cancer
    Year 2022
    Journal Cell Death & Disease
    Authors Zhang Dan, et al.
    Applications Unspecified
    Reactivity
  • Regulation of Cellular Metabolism by Protein Crotonylation Coordinates Pancreatic Cancer Progression
    Year 2022
    Journal Cell Reports
    Authors Zheng Yan, et al.
    Applications Unspecified
    Reactivity
  • Involvement of Histone Lysine Crotonylation in the Regulation of Nerve-Injury-Induced Neuropathic Pain
    Year 2022
    Journal Frontiers in Immunology
    Authors Yu Zou, et al.
    Applications Unspecified
    Reactivity
  • Sorafenib Attenuates Fibrotic Hepatic Injury Through Mediating Lysine Crotonylation
    Year 2022
    Journal Drug Design Development and Therapy
    Authors Xiao-Feng Chen, et al.
    Applications Unspecified
    Reactivity
  • CDYL1-dependent decrease in lysine crotonylation at DNA double-strand break sites functionally uncouples transcriptional silencing and repair
    Year 2022
    Journal MOLECULAR CELL
    Authors Enas R. Abu-Zhayia, et al.
    Applications Unspecified
    Reactivity
  • Acox2 is a regulator of lysine crotonylation that mediates hepatic metabolic homeostasis in mice
    Year 2022
    Journal Cell Death & Disease
    Authors Zhang Yuan, et al.
    Applications Unspecified
    Reactivity
  • Class I histone deacetylases (HDAC1–3) are histone lysine delactylases
    Year 2022
    Journal Science Advances
    Authors Carlos Moreno-Yruela, et al.
    Applications Unspecified
    Reactivity
  • The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum
    Year 2021
    Journal eLife
    Authors Ning Zhang, et al.
    Applications Unspecified
    Reactivity
  • Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis
    Year 2021
    Journal Nature Chemical Biology
    Authors Song Xiaoyu, et al.
    Applications Unspecified
    Reactivity
  • LncRNA NEAT1 induces autophagy through epigenetic regulation of autophagy-related gene expression in neuroglial cells
    Year 2021
    Journal JOURNAL OF CELLULAR PHYSIOLOGY
    Authors Ziqiang Wang, et al.
    Applications Unspecified
    Reactivity
  • Epigenetics Identifier screens reveal regulators of chromatin acylation and limited specificity of acylation antibodies
    Year 2021
    Journal Scientific Reports
    Authors Kollenstart Leonie, et al.
    Applications Unspecified
    Reactivity
  • Tsga8 is required for spermatid morphogenesis and male fertility in mice
    Year 2021
    Journal DEVELOPMENT
    Authors Yuki Kobayashi, et al.
    Applications Unspecified
    Reactivity
  • Global Profiling of the Lysine Crotonylome in Different Pluripotent States
    Year 2021
    Journal GENOMICS PROTEOMICS & BIOINFORMATICS
    Authors Yuan Lv, et al.
    Applications Unspecified
    Reactivity
  • Histone crotonylation promotes mesoendodermal commitment of human embryonic stem cells
    Year 2021
    Journal Cell Stem Cell
    Authors Yi Fang, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence
    Year 2021
    Journal Journal of Integrative Plant Biology
    Authors Xiaoyang Chen, et al.
    Applications Unspecified
    Reactivity
  • p300-Catalyzed Lysine Crotonylation Promotes the Proliferation, Invasion, and Migration of HeLa Cells via Heterogeneous Nuclear Ribonucleoprotein A1
    Year 2020
    Journal Analytical Cellular Pathology
    Authors Xuesong Han, et al.
    Applications Unspecified
    Reactivity
  • Crotonylation of key metabolic enzymes regulates carbon catabolite repression in Streptomyces roseosporus
    Year 2020
    Journal Communications Biology
    Authors Sun Chen-Fan, et al.
    Applications Unspecified
    Reactivity
  • The YEATS Domain Histone Crotonylation Readers Control Virulence-Related Biology of a Major Human Pathogen
    Year 2020
    Journal Cell Reports
    Authors Qi Wang, et al.
    Applications Unspecified
    Reactivity
  • Crotonylation at serine 46 impairs p53 activity
    Year 2020
    Journal BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    Authors Peng Liao, et al.
    Applications Unspecified
    Reactivity
  • Gcn5 and Esa1 function as histone crotonyltransferases to regulate crotonylation-dependent transcription
    Year 2019
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Leonie Kollenstart, et al.
    Applications Unspecified
    Reactivity
  • Lysine glutarylation in human sperm is associated with progressive motility
    Year 2019
    Journal HUMAN REPRODUCTION
    Authors Cheng Yi-min, et al.
    Applications Unspecified
    Reactivity
  • NEAT1 regulates neuroglial cell mediating Aβ clearance via the epigenetic regulation of endocytosis-related genes expression
    Year 2019
    Journal CELLULAR AND MOLECULAR LIFE SCIENCES
    Authors Wang Ziqiang, et al.
    Applications Unspecified
    Reactivity
  • MiR-155-5p modulates HSV-1 replication via the epigenetic regulation of SRSF2 gene expression
    Year 2019
    Journal Epigenetics
    Authors Ziqiang Wang, et al.
    Applications Unspecified
    Reactivity
  • HDAC-dependent decrease in histone crotonylation during DNA damage
    Year 2019
    Journal Journal of Molecular Cell Biology
    Authors Abu-Zhayia Enas R, et al.
    Applications Unspecified
    Reactivity
  • Investigation of the Developmental Requirements of Drosophila HP1 and Insulator Protein Partner, HIPP1
    Year 2019
    Journal G3-Genes Genomes Genetics
    Authors Glenn Steve E, et al.
    Applications Unspecified
    Reactivity
  • Lysine crotonylation is involved in hepatocellular carcinoma progression
    Year 2019
    Journal BIOMEDICINE & PHARMACOTHERAPY
    Authors Junhu Wan, et al.
    Applications Unspecified
    Reactivity
  • Dynamics and functional interplay of histone lysine butyrylation, crotonylation, and acetylation in rice under starvation and submergence
    Year 2018
    Journal GENOME BIOLOGY
    Authors Lu Yue, et al.
    Applications Unspecified
    Reactivity
  • Nejire/dCBP-mediated histone H3 acetylation during spermatogenesis is essential for male fertility in Drosophila melanogaster
    Year 2018
    Journal PLoS One
    Authors Tim Hundertmark, et al.
    Applications Unspecified
    Reactivity
  • Global Involvement of Lysine Crotonylation in Protein Modification and Transcription Regulation in Rice
    Year 2018
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Shuai Liu, et al.
    Applications Unspecified
    Reactivity
  • Quantitative Crotonylome Analysis Expands the Roles of p300 in the Regulation of Lysine Crotonylation Pathway
    Year 2018
    Journal PROTEOMICS
    Authors He Huang, et al.
    Applications Unspecified
    Reactivity
  • p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis
    Year 2018
    Journal MOLECULAR CELL
    Authors He Huang, et al.
    Applications Unspecified
    Reactivity
  • First profiling of lysine crotonylation of myofilament proteins and ribosomal proteins in zebrafish embryos
    Year 2018
    Journal Scientific Reports
    Authors Kwon Oh Kwang, et al.
    Applications Unspecified
    Reactivity
  • Microbiota derived short chain fatty acids promote histone crotonylation in the colon through histone deacetylases
    Year 2018
    Journal Nature Communications
    Authors Fellows Rachel, et al.
    Applications Unspecified
    Reactivity
  • Characterization of histone acylations links chromatin modifications with metabolism
    Year 2017
    Journal Nature Communications
    Authors Simithy Johayra, et al.
    Applications Unspecified
    Reactivity
  • Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis
    Year 2017
    Journal MOLECULAR CELL
    Authors Shumeng Liu, et al.
    Applications Unspecified
    Reactivity
  • MOF as an evolutionarily conserved histone crotonyltransferase and transcriptional activation by histone acetyltransferase-deficient and crotonyltransferase-competent CBP/p300
    Year 2017
    Journal Cell Discovery
    Authors Liu Xiaoguang, et al.
    Applications Unspecified
    Reactivity
  • Class I histone deacetylases are major histone decrotonylases: evidence for critical and broad function of histone crotonylation in transcription
    Year 2017
    Journal CELL RESEARCH
    Authors Wei Wei, et al.
    Applications Unspecified
    Reactivity
  • Large-Scale Identification of Protein Crotonylation Reveals Its Role in Multiple Cellular Functions
    Year 2017
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Wei Wei, et al.
    Applications Unspecified
    Reactivity
  • Histone lysine crotonylation during acute kidney injury in mice
    Year 2016
    Journal Disease Models & Mechanisms
    Authors Olga Ruiz-Andres, et al.
    Applications Unspecified
    Reactivity
  • Poised chromatin and bivalent domains facilitate the mitosis-to-meiosis transition in the male germline
    Year 2015
    Journal BMC BIOLOGY
    Authors Sin Ho-Su, et al.
    Applications Unspecified
    Reactivity
  • Intracellular Crotonyl-CoA Stimulates Transcription through p300-Catalyzed Histone Crotonylation
    Year 2015
    Journal MOLECULAR CELL
    Authors Benjamin R. Sabari, et al.
    Applications Unspecified
    Reactivity
  • Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark
    Year 2014
    Journal Nature Chemical Biology
    Authors Dai Lunzhi, et al.
    Applications Unspecified
    Reactivity
  • RNF8 regulates active epigenetic modifications and escape gene activation from inactive sex chromosomes in post-meiotic spermatids
    Year 2012
    Journal GENES & DEVELOPMENT
    Authors Ho-Su Sin, et al.
    Applications Unspecified
    Reactivity
  • Site-Specific Incorporation of ε-N-Crotonyllysine into Histones
    Year 2012
    Journal ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
    Authors Chan Hyuk Kim, et al.
    Applications Unspecified
    Reactivity