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Anti-D-Lactyllysine Rabbit mAb
Catalog Number: PTM-1429RM
$ 420

Clone Number: CAPTM-O0804

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: Kla, D-la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kla, D-la
UniProt ID

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Immunogen D-lactylated lysine peptides
MW (kDa) Multiple
Specificity Anti-D-Lactyl Lysine Rabbit mAb detects proteins with D-lactylated lysine residues, with no cross-reactivity to its isomers L-lactyl Lysine. This pan antibody recognizes D-lactylated lysine regardless of the surrounding amino acid sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

D-lactyl lysine (KD-la) refers to the addition of D-lactate to lysine residues in proteins. This modification was first reported by Professor Yingming Zhao's team at the University of Chicago in 2024, with the findings published in Nature Chemical Biology. KD-la is formed by an uncatalyzed reaction between proteins and S-d-lactoylglutathione (LGSH), which is produced through the glyoxalase pathway. The glyoxalase pathway involves two enzymes: glyoxalase 1 (GLO1) and glyoxalase 2 (GLO2). GLO1 conjugates methylglyoxal (MGO), a byproduct of glycolysis, to glutathione to form LGSH, which is then hydrolyzed by GLO2 to produce D-lactate and regenerate cellular glutathione. In humans, L-lactate is a ubiquitous metabolic product that plays a key role in energy metabolism, especially during muscle activity. In contrast, D-lactate is not a product of normal human metabolism but is primarily produced by certain microorganisms such as yeasts and some bacteria during fermentation. The potential harmfulness of D-lactate to the human body mainly lies in its accumulation under specific conditions, particularly in cases of intestinal microbiota dysbiosis, short bowel syndrome, or certain disease states. Excessive production of D-lactate can lead to Dlactic acidosis, which may present symptoms of acidosis such as low blood bicarbonate levels, decreased blood pH, and hyperuricemia. In severe cases, it can cause neurological symptoms such as seizures, ataxia, and altered consciousness. This antibody has undergone rigorous specificity validation and binds exclusively to lysine KD-la, without cross-reactivity with its isomers lysine KL-la and Kce.

Cellular location

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Images
Dot Blot

Peptide amount: 4 ng, 20 ng, 50 ng, 200ng
Blocking buffer: 3% BSA/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: D-lactylated peptide library. Lane 2: L-lactylated peptide library.
Lane 3: carboxyethylated peptide library. Lane 4: acetylated peptide library.
Lane 5: butyrylated peptide library. Lane 6: crotonylated peptide library.
Lane 7: 2-hydroxyisobutyrylated peptide library. Lane 8: succinylated peptide library.
Lane 9: unmodified peptide library.

WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Lysates: (-): HepG2 cells; (+): HepG2 cells + Serum (0%, 47 hours) add sodium D-lactate (100 mM, 23 hours) add trichostatin A (1 µm, 5 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Research Use

For research use only, not for use in diagnostic procedures.