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Anti-DDX4 Rabbit pAb
Catalog Number: PTM-6904
$ 300

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB IHC-P

Reactivity: Human, Rat

Synonyms: VASA

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms VASA
UniProt ID

Q9NQI0

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Rat
IHC-P 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells. This enzyme is specific to germ cells and is necessary for germ cell development. Mouse DDX4 was shown to interact with Dicer, suggesting a role in microRNA-mediated RNA silencing.

Cellular location

Cytoplasm, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1:Rat ovary
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 79 kDa
Observed band size: 79 kDa

IHC-P

Tissue: Human testis
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6904

Research Use

For research use only, not for use in diagnostic procedures.

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    Recombinant Rabbit Monoclonal
    Anti-DDX4 Rabbit mAb
    Catalog #
    PTM-6370
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P