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Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-1104
$ 1,920

Clone Number: /

Host: / Clonality: /

Applications： IAP

Reactivity: All

Synonyms: K-ε-GG

Product Size
500 μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms K-ε-GG
UniProt ID

/

Immunogen Diglycylated lysine peptides
MW (kDa) Multiple
Specificity With the immobilization of highly specific Anti-Diglycyl-Lysine Antibody, the Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads selectively capture peptides/proteins bearing K-ɛ-G-G residues, which are remnants of ubiquitin left in protein substrates after trypsin digestion or remnants of SUMO after α-lytic protease WaLP digestion. This product has been well utilized to affinity purify peptides for global proteomic screening of ubiquitination and SUMOylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminus and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule. Members of the ubiquitin-like protein family, such as small ubiquitin-related modifier 1, 2 and 3 (SUMO1, 2 and 3), have functions similar to ubiquitin, whereby they bind to target proteins as part of a post-translational modification system. SUMO can be covalently attached to proteins as a monomer or a lysine-linked polymer via an isopeptide bond. SUMO-1 regulates nuclear trafficking, formation of subnuclear structures, transcriptional activity, and protein stability by being conjugated to proteins such as RanGAP, PML, p53 and IκB-α. SUMO-2/-3 forms poly-(SUMO) chains and is conjugated to topoisomerase II and APP, regulating chromosomal segregation and cellular responses to environmental stress.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-Diglycyl-Lysine Rabbit pAb (PTM-1101) on modified and unmodiifed peptides/proteins.
Peptide/Protein amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides and proteins used in the experiment is provided in the table below.
Lane 1: K-ε-GG-KLH. Lane 2: K-ε-GG library.
Lane 3: Synthetic unmodified human histone H3 peptide STGGKAPR (aa 10-17).
Lane 4: KLH. Lane 5: Control peptides.

Research Use

For research use only, not for use in diagnostic procedures.

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    Year 2023
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    Applications Unspecified
    Reactivity
  • An integrated multi-omics analysis of identifies distinct molecular characteristics in pulmonary infections of Pseudomonas aeruginosa
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    Reactivity
  • SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation
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    Reactivity
  • Establishment of a Landscape of UPL5-Ubiquitinated on Multiple Subcellular Components of Leaf Senescence Cell in Arabidopsis
    Year 2022
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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    Applications Unspecified
    Reactivity
  • Root Membrane Ubiquitinome under Short-Term Osmotic Stress
    Year 2022
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Nathalie Berger, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive Analysis of Ubiquitome Changes in Nicotiana benthamiana after Rice Stripe Virus Infection
    Year 2022
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    Applications Unspecified
    Reactivity
  • Central Role of Ubiquitination in Wheat Response to CWMV Infection
    Year 2022
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    Authors Haichao Hu, et al.
    Applications Unspecified
    Reactivity
  • The Analysis of the Ubiquitylomic Responses to Streptococcus agalactiae Infection in Bovine Mammary Gland Epithelial Cells
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    Applications Unspecified
    Reactivity
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    Year 2022
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    Reactivity
  • Global analysis of HBV-mediated host proteome and ubiquitylome change in HepG2.2.15 human hepatoblastoma cell line
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    Reactivity
  • Serpinc1 Acts as a Tumor Suppressor in Hepatocellular Carcinoma Through Inducing Apoptosis and Blocking Macrophage Polarization in an Ubiquitin-Proteasome Manner
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    Reactivity
  • Protein Modification Characteristics of the Malaria Parasite Plasmodium falciparum and the Infected Erythrocytes
    Year 2021
    Journal MOLECULAR & CELLULAR PROTEOMICS
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    Reactivity
  • Nitrogen-Regulated Theanine and Flavonoid Biosynthesis in Tea Plant Roots: Protein-Level Regulation Revealed by Multiomics Analyses
    Year 2021
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    Reactivity
  • Ubiquitin-Modified Proteome of SARS-CoV-2-Infected Host Cells Reveals Insights into Virus–Host Interaction and Pathogenesis
    Year 2021
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Huan Zhang, et al.
    Applications Unspecified
    Reactivity
  • Identification of ubiquitinated substrate proteins and their gene expression patterns in lung adenocarcinoma
    Year 2021
    Journal Annals of Translational Medicine
    Authors Ran Xu, et al.
    Applications Unspecified
    Reactivity
  • Brucella melitensis UGPase inhibits the activation of NF-κB by modulating the ubiquitination of NEMO
    Year 2021
    Journal BMC Veterinary Research
    Authors Zhou Yucheng, et al.
    Applications Unspecified
    Reactivity
  • Ubiquitinome Profiling Reveals the Landscape of Ubiquitination Regulation in Rice Young Panicles
    Year 2020
    Journal GENOMICS PROTEOMICS & BIOINFORMATICS
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    Applications Unspecified
    Reactivity
  • Pleiotropic effects of Ubi4, a polyubiquitin precursor required for ubiquitin accumulation, conidiation and pathogenicity of a fungal insect pathogen
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    Journal ENVIRONMENTAL MICROBIOLOGY
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  • Comparative Ubiquitome Analysis under Heat Stress Reveals Diverse Functions of Ubiquitination in Saccharina japonica
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    Reactivity
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    Reactivity
  • Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis
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    Journal BIOSCIENCE REPORTS
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    Applications Unspecified
    Reactivity
  • Comprehensive Proteomic Analysis of Lysine Ubiquitination in Seedling Leaves of Nicotiana tabacum
    Year 2020
    Journal ACS Omega
    Authors Huaixu Zhan, et al.
    Applications Unspecified
    Reactivity
  • Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis)
    Year 2020
    Journal MOLECULAR REPRODUCTION AND DEVELOPMENT
    Authors Peng‐fei Zhang, et al.
    Applications Unspecified
    Reactivity
  • Ubiquitylome study identifies increased histone 2A ubiquitylation as an evolutionarily conserved aging biomarker
    Year 2019
    Journal Nature Communications
    Authors Yang Lu, et al.
    Applications Unspecified
    Reactivity
  • Quantitative ubiquitylomics approach for characterizing the dynamic change and extensive modulation of ubiquitylation in rice seed germination
    Year 2019
    Journal PLANT JOURNAL
    Authors Dongli He, et al.
    Applications Unspecified
    Reactivity
  • Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia ‘He Xie’
    Year 2019
    Journal JOURNAL OF EXPERIMENTAL BOTANY
    Authors Gu Zhaoyu, et al.
    Applications Unspecified
    Reactivity
  • Proteome and Ubiquitome Changes during Rose Petal Senescence
    Year 2019
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Jingyun Lu, et al.
    Applications Unspecified
    Reactivity
  • Marek’s Disease Virus Regulates the Ubiquitylome of Chicken CD4+ T Cells to Promote Tumorigenesis
    Year 2019
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Xiaolu Zhou, et al.
    Applications Unspecified
    Reactivity
  • Comprehensive analysis of the ubiquitome in rabies virus-infected brain tissue of Mus musculus
    Year 2019
    Journal VETERINARY MICROBIOLOGY
    Authors Yuchen Cai, et al.
    Applications Unspecified
    Reactivity
  • Global ubiquitome analysis of substantia nigra in doubly-mutant human alpha-synuclein transgenic mice
    Year 2019
    Journal BEHAVIOURAL BRAIN RESEARCH
    Authors Feng He, et al.
    Applications Unspecified
    Reactivity
  • Ubiquitylomes Analysis of the Whole blood in Postmenopausal Osteoporosis Patients and healthy Postmenopausal Women
    Year 2019
    Journal Orthopaedic Surgery
    Authors Yi-ran Yang, et al.
    Applications Unspecified
    Reactivity
  • cpubi4 Is Essential for Development and Virulence in Chestnut Blight Fungus
    Year 2018
    Journal Frontiers in Microbiology
    Authors Chen Qi, et al.
    Applications Unspecified
    Reactivity
  • Global analysis of ubiquitome in PRRSV-infected pulmonary alveolar macrophages
    Year 2018
    Journal Journal of Proteomics
    Authors Huan Zhang, et al.
    Applications Unspecified
    Reactivity
  • Integrative Analysis of Proteome and Ubiquitylome Reveals Unique Features of Lysosomal and Endocytic Pathways in Gefitinib-Resistant Non-Small Cell Lung Cancer Cells
    Year 2018
    Journal PROTEOMICS
    Authors Wang Li, et al.
    Applications Unspecified
    Reactivity
  • Quantitative ubiquitylome analysis and crosstalk with proteome/acetylome analysis identified novel pathways and targets of perifosine treatment in neuroblastoma
    Year 2018
    Journal Translational Cancer Research
    Authors Min Jiang, et al.
    Applications Unspecified
    Reactivity
  • Qualitative ubiquitome unveils the potential significances of protein lysine ubiquitination in hyphal growth of Aspergillus nidulans
    Year 2015
    Journal CURRENT GENETICS
    Authors Chu Xin-Ling, et al.
    Applications Unspecified
    Reactivity
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