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Recombinant Rabbit Monoclonal
Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-1104
$ 1,920

Clone Number: /

Host: / Clonality: /

Applications: IAP

Reactivity: All

Synonyms: K-ε-GG

Product Size
500μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms K-ε-GG
UniProt ID

/

Immunogen Diglycylated lysine peptides
MW (kDa) Multiple
Specificity With the immobilization of highly specific Anti-Diglycyl-Lysine Antibody, the Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads selectively capture peptides/proteins bearing K-ɛ-G-G residues, which are remnants of ubiquitin left in protein substrates after trypsin digestion or remnants of SUMO after α-lytic protease WaLP digestion. This product has been well utilized to affinity purify peptides for global proteomic screening of ubiquitination and SUMOylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminus and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule. Members of the ubiquitin-like protein family, such as small ubiquitin-related modifier 1, 2 and 3 (SUMO1, 2 and 3), have functions similar to ubiquitin, whereby they bind to target proteins as part of a post-translational modification system. SUMO can be covalently attached to proteins as a monomer or a lysine-linked polymer via an isopeptide bond. SUMO-1 regulates nuclear trafficking, formation of subnuclear structures, transcriptional activity, and protein stability by being conjugated to proteins such as RanGAP, PML, p53 and IκB-α. SUMO-2/-3 forms poly-(SUMO) chains and is conjugated to topoisomerase II and APP, regulating chromosomal segregation and cellular responses to environmental stress.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-Diglycyl-Lysine Rabbit pAb (PTM-1101) on modified and unmodiifed peptides/proteins.
Peptide/Protein amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides and proteins used in the experiment is provided in the table below.
Lane 1: K-ε-GG-KLH. Lane 2: K-ε-GG library.
Lane 3: Synthetic unmodified human histone H3 peptide STGGKAPR (aa 10-17).
Lane 4: KLH. Lane 5: Control peptides.

Research Use

For research use only, not for use in diagnostic procedures.

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  • An integrated multi-omics analysis of identifies distinct molecular characteristics in pulmonary infections of Pseudomonas aeruginosa
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  • Pleiotropic effects of Ubi4, a polyubiquitin precursor required for ubiquitin accumulation, conidiation and pathogenicity of a fungal insect pathogen
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    Journal MOLECULAR REPRODUCTION AND DEVELOPMENT
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  • Ubiquitylome study identifies increased histone 2A ubiquitylation as an evolutionarily conserved aging biomarker
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    Journal Nature Communications
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  • Quantitative ubiquitylomics approach for characterizing the dynamic change and extensive modulation of ubiquitylation in rice seed germination
    Year 2019
    Journal PLANT JOURNAL
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  • Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia ‘He Xie’
    Year 2019
    Journal JOURNAL OF EXPERIMENTAL BOTANY
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  • Proteome and Ubiquitome Changes during Rose Petal Senescence
    Year 2019
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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  • Marek’s Disease Virus Regulates the Ubiquitylome of Chicken CD4+ T Cells to Promote Tumorigenesis
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  • Comprehensive analysis of the ubiquitome in rabies virus-infected brain tissue of Mus musculus
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    Journal VETERINARY MICROBIOLOGY
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  • Global ubiquitome analysis of substantia nigra in doubly-mutant human alpha-synuclein transgenic mice
    Year 2019
    Journal BEHAVIOURAL BRAIN RESEARCH
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  • Ubiquitylomes Analysis of the Whole blood in Postmenopausal Osteoporosis Patients and healthy Postmenopausal Women
    Year 2019
    Journal Orthopaedic Surgery
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  • cpubi4 Is Essential for Development and Virulence in Chestnut Blight Fungus
    Year 2018
    Journal Frontiers in Microbiology
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  • Global analysis of ubiquitome in PRRSV-infected pulmonary alveolar macrophages
    Year 2018
    Journal Journal of Proteomics
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  • Integrative Analysis of Proteome and Ubiquitylome Reveals Unique Features of Lysosomal and Endocytic Pathways in Gefitinib-Resistant Non-Small Cell Lung Cancer Cells
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    Journal PROTEOMICS
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  • Quantitative ubiquitylome analysis and crosstalk with proteome/acetylome analysis identified novel pathways and targets of perifosine treatment in neuroblastoma
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    Journal Translational Cancer Research
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  • Qualitative ubiquitome unveils the potential significances of protein lysine ubiquitination in hyphal growth of Aspergillus nidulans
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