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Rabbit Monoclonal
Anti-Dimethyllysine Rabbit mAb (BSA and Azide Free)
Catalog Number: PTM-606F
$ 495

Clone Number: PT-O0403

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: All

Synonyms: Kme2

Product Size
100 μg
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kme2
UniProt ID

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Immunogen Dimethylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Methyl-Lysine Rabbit mAb (BSA and Azide Free) detects proteins with dimethylated lysine residues. This pan antibody recognizes dimethylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 All
Properties
Purity Protein A
Constituents PBS
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone methylation primarily occurs at lysine and arginine residues on the amino terminus of core histones. The methylation of histones can either enhance or repress gene transcription, depending on the specific amino acids (Lys or Arg) being methylated and the number of attached methyl groups (mono-, di-, or tri-methylation for Lys; mono-di-symmetric/asymmetric methylation for Arg). Lysine methylation is commonly observed at histone H3 Lys residues 4, 9, 27, 36, 79, and histone H4 Lys20, while Arginine methylation predominantly occurs at histone H3 Arg residues 2, 8, 17, 26, and histone H4 Arg3. The regulation of histone methylation is primarily governed by histone methylases (HMTs) and histone demethylases (HDMs).

Cellular location

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Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Peptide quantity: 4 ng, 20 ng, 50ng, 100 ng
Exposure time: 60 s
The list of peptides is included below:
Lane 1 Kme2 library. Lane 2 Kme library.
Lane 3 Kme library. Lane 4 Kme3 library.
Lane 5 Kac library. Lane 6 Library control.

WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: Neuro-2a, 3: BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: Multiple kDa
Observed MW: Multiple kDa

Research Use

For research use only, not for use in diagnostic procedures.