Clone Number: 13762
Host: Mouse Clonality: Recombinant Monoclonal
Applications： WB IP ChIP
Reactivity: All
Synonyms: /
Shipping: Ambient temperature
Order online or send purchase order to info@ptmbio.com
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Synonyms | / |
| UniProt ID | |
| Immunogen | Synthetic DYKDDDDK peptide |
| MW (kDa) | / |
| Specificity | Anti-DYKDDDDK Tag Mouse mAb recognizes the DYKDDDDK epitope on tagged recombinant proteins. It binds to this linear epitope independent of the fusion partner’s N- or C-terminal placement. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:5000 - 1:20000 | All |
| IP | 1:25 - 1:100 | All |
| ChIP | 6 μg per 2x106 cells | All |
| Purity | Protein G purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
The DYKDDDDK epitope tag, also known as the FLAG® tag (registered trademark of Sigma-Aldrich) is an eight-amino acid peptide sequence that can be fused to a protein of interest. This tag allows for the detection and purification of the tagged protein with minimal disruption to the tagged protein's function.
Cellular location
/
| WB | |
|---|---|
|
|
Lysates: 1. HEK-293T cells transfected with an empty vector (vector control), whole cell lysate; 2. HEK-293T cells transfected with a PFKFB3 (WT) expression vector (58 kDa) containing a DYKDDDDK-tag, whole cell lysate |
| IP | |
|---|---|
|
|
IP of whole cell lysates from HEK-293T cells transfected with a DYKDDDDK-tagged PFKFB3 (WT) expression vector |
| ChIP | |
|---|---|
|
|
Sample: 293F cells transfected with a DYKDDDDK-tagged FLAG-MAX expression vector (18 kDa) |
Research Use
For research use only, not for use in diagnostic procedures.
