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Recombinant Rabbit Monoclonal
Anti-EHMT1 Rabbit mAb
Catalog Number: PTM-6205
$ 290

Clone Number: 12734

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: GLP, GLP1, EUHMTASE1, GLP, KIAA1876, KMT1D

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms GLP, GLP1, EUHMTASE1, GLP, KIAA1876, KMT1D
UniProt ID

Q9H9B1

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:2000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me).

Cellular location

Chromosome, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: MCF7, 2: T-47D, 3: SK-BR-3
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 141 kDa
Observed band size: 180 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 141 kDa
Observed band size: 180 kDa

Research Use

For research use only, not for use in diagnostic procedures.