/ EN
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
/ EN
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-EHMT1 Rabbit mAb
Catalog Number: PTM-6205
$ 290

Clone Number: 12734

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: Histone-lysine N-methyltransferase EHMT1, Euchromatic histone-lysine N-methyltransferase 1 (Eu-HMTase1), G9a-like protein 1 (GLP; GLP1), Histone H3-K9 methyltransferase 5 (H3-K9-HMTase 5), Lysine N-methyltransferase 1D, EUHMTASE1, GLP, KIAA1876, KMT1D

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Histone-lysine N-methyltransferase EHMT1, Euchromatic histone-lysine N-methyltransferase 1 (Eu-HMTase1), G9a-like protein 1 (GLP; GLP1), Histone H3-K9 methyltransferase 5 (H3-K9-HMTase 5), Lysine N-methyltransferase 1D, EUHMTASE1, GLP, KIAA1876, KMT1D
UniProt ID

Q9H9B1

Immunogen Synthetic peptide derived from human histone-lysine N-methyltransferase EHMT1
MW (kDa) 141
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:2000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me).

Cellular location

Chromosome, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: MCF7, 2: T-47D, 3: SK-BR-3
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 141 kDa
Observed band size: 180 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 141 kDa
Observed band size: 180 kDa

Research Use

For research use only, not for use in diagnostic procedures.