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Anti-ENO1 Rabbit pAb
Catalog Number: PTM-6780
$ 280

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: Alpha-enolase, Enolase 1, MBP-1, MPB-1, NNE, ENO1L1, MBPB1

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Alpha-enolase, Enolase 1, MBP-1, MPB-1, NNE, ENO1L1, MBPB1
UniProt ID


MW (kDa)
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:5000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Human
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information


ENO1, also named as NNE, ENO1L1, MBPB1 and MPB1, belongs to the enolase family. It is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. ENO1 may also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. It stimulates immunoglobulin production.

Cellular location

Nucleus, cytoplasm, cell membrane


Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate)
Lysate: 1: MCF-7, 2: HeLa, 3: Human glioma, 4: Mouse brain, 5: Rat brain
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted band size: 47 kDa
Observed band size: 47 / 62 / 92 kDa


Tissue: Human pancreas
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6780

Research Use

For research use only, not for use in diagnostic procedures.