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Recombinant
Anti-ENO3 Mouse mAb
Catalog Number: PTM-6021
$ 280

Clone Number: 12200

Host: Mouse Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF

Reactivity: Human, Rat

Synonyms: beta-enolase

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG3/kappa
Conjugate Unconjugated
Synonyms beta-enolase
UniProt ID

P13929

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Rat
IHC-P 1:50 - 1:100 Human
ICC/IF 1:50 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Appears to have a function in striated muscle development and regeneration.

Cellular location

Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, Raji, HepG2, Rat skeletal muscla
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 52 kDa
Observed band size: 52 kDa
IHC-P

Tissue: Human pancreas
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6021

Tissue: Human skeletal muscle
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6021
ICC/IF

Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-6021

Research Use

For research use only, not for use in diagnostic procedures.

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  • View More Images（5）
    $ 290
    Anti-ENO3 Rabbit pAb
    Catalog #
    PTM-6731
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (1)
  • Circular RNA circMYLK4 shifts energy metabolism from glycolysis to OXPHOS by binding to the calcium channel auxiliary subunit CACNA2D2
    Year 2024
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Haigang Cao, et al.
    Applications Unspecified
    Reactivity
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