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Recombinant Rabbit Monoclonal
Anti-EZH2 Rabbit mAb
Catalog Number: PTM-5227
$ 290

Clone Number: JRMR-204

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: ENX-1, KMT6, Enhancer of zeste homolog 2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms ENX-1, KMT6, Enhancer of zeste homolog 2
UniProt ID

Q15910

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse
IHC-P 1:200 - 1:1000 Human, Mouse, Rat
ICC/IF 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, Jurkat, MCF7, Neuro-2a, 3T3
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 85 kDa
Observed band size: 90 kDa
IHC-P

Tissue: Human cervical carcinoma
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5227

Tissue: Mouse spleen
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5227

Tissue: Mouse testis
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5227

Tissue: Rat spleen
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5227

Tissue: Rat testis
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5227

Research Use

For research use only, not for use in diagnostic procedures.

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