Clone Number: 13762
Host: Mouse Clonality: Recombinant Monoclonal
Applications： WB IP ChIP
Reactivity: /
Synonyms: /
Shipping: Ambient temperature
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| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Synonyms | / |
| UniProt ID | |
| Immunogen | |
| MW (kDa) | 58 |
| Specificity | Anti-DYKDDDDK Tag Mouse mAb recognizes endogeneous level of DYKDDDDK Tag protein. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:5000 - 1:20000 | / |
| IP | 1:25 - 1:100 | / |
| ChIP | 6 μg/2x106 cells | / |
| Purity | Protein G purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
The DYKDDDDK epitope tag, also known as the FLAG® tag (registered trademark of Sigma-Aldrich) is an eight-amino acid peptide sequence that can be fused to a protein of interest. This tag allows for the detection and purification of the tagged protein with minimal disruption to the tagged protein's function.
Cellular location
/
| WB | |
|---|---|
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Blocking buffer: 5% NFDM/TBST |
| IP | |
|---|---|
|
IP of HEK-293T transfected with PFKFB3 (WT) (60 kDa) expression vector containing a Flag-tag cells extracts |
| ChIP | |
|---|---|
|
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Cell type: 293F transfected with FLAG-MAX (18 |
Research Use
For research use only, not for use in diagnostic procedures.
