Clone Number: JRMR-10034-3
Host: Rabbit Clonality: Recombinant Monoclonal
Applications： WB IP ChIP
Reactivity: All
Synonyms: /
Shipping: Ambient temperature
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| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | / |
| UniProt ID | |
| Immunogen | Synthetic DYKDDDDK peptide |
| MW (kDa) | / |
| Specificity | Anti-DYKDDDDK Tag Rabbit mAb recognizes exogenously expressed DYKDDDDK-tagged proteins. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:10000 - 1:100000 | / |
| IP | 1:100 | / |
| ChIP | 6 μg per 2x106 cells | / |
| Purity | Protein A purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
The DYKDDDDK epitope tag, also known as the FLAG® tag (registered trademark of Sigma-Aldrich) is an eight-amino acid peptide sequence that can be fused to a protein of interest. This tag allows for the detection and purification of the tagged protein with minimal disruption to the tagged protein's function.
Cellular location
/
| WB | |
|---|---|
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Lysates: 1. HEK-293T human embryonic kidney cells transfected with an empty vector (vector control), whole cell lysate; 2. HEK-293T transfected with PFKFB3 (WT) expression vector containing a DYKDDDDK tag, whole cell lysate |
| IP | |
|---|---|
|
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IP of 293T transfected with FLAG-MAX expression vector (18kDa) containing DYKDDDDK tag |
| ChIP | |
|---|---|
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Sample: 293F cells transfected with FLAG-MAX expression vector (18kDa) containing DYKDDDDK tag |
Research Use
For research use only, not for use in diagnostic procedures.
