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Recombinant
Anti-GAPDH Mouse mAb
Catalog Number: PTM-5150
$ 125

Clone Number: 12113

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Mouse, Rat

Synonyms: GAPD

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1/kappa
Conjugate Unconjugated
Synonyms GAPD
UniProt ID

P04406

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated GAPDH) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.

Cellular location

Cytoplasm, Cytoskeleton, Membrane, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa, 2: Jurkat, 3: NIH/3T3, 4: BRL
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 36 kDa
Observed band size: 37 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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References (1)
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