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Recombinant
Anti-GAPDH Mouse mAb
Catalog Number: PTM-5150
References (7)
$ 125

Clone Number: 12113

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Mouse, Rat

Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD
UniProt ID

P04406

Immunogen Synthetic peptide derived from human GAPDH
MW (kDa) 36
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated GAPDH) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.

Cellular location

Cytoplasm, Cytoskeleton, Membrane, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa, 2: Jurkat, 3: NIH/3T3, 4: BRL
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 36 kDa
Observed band size: 37 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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References (7)
  • Lactylome Reprogramming Mediates Therapeutic Response and Adaptation to Neoadjuvant Chemotherapy in Esophageal Squamous Cell Carcinoma
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  • Lysine pyruvylation couples glycolytic flux to epigenetic regulation
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  • Anti-EGFR therapy can overcome acquired resistance to the third-generation ALK-tyrosine kinase inhibitor lorlatinib mediated by activation of EGFR
    Year 2025
    Journal Acta Pharmacol Sin
    Authors Chen Hu, et al.
    Applications Unspecified
    Reactivity
  • Plasmodium berghei IMC1j interacts with δ-tubulin to orchestrate subpellicular microtubule organization in ookinetes
    Year 2025
    Journal mBio
    Authors Gang He, et al.
    Applications Unspecified
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  • ULK1-dependent phosphorylation of OGT instructs the tumorigenicity of O-GlcNAcylation
    Year 2025
    Journal Sci China Life Sci
    Authors Zhuan Lv, et al.
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  • CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancer
    Year 2024
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    Authors Cong Xiang, et al.
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  • Jellyfish stings-induced cardiac failure was ameliorated through AAG-mediated glycogen-driven ATP production
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    Journal Exploration (Beijing)
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