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Recombinant
Anti-GAPDH Mouse mAb
Catalog Number: PTM-6684
$ 125

Clone Number: JMMR-2818

Host: Mouse Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF FC

Reactivity: Human, Mouse, Rat, A.thaliana

Synonyms: GAPD

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2b
Conjugate Unconjugated
Synonyms GAPD
UniProt ID

P04406

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:10000 Human, Mouse, Rat, Arabidopsis thaliana
IHC-P 1:50 - 1:100 Human, Mouse, Rat
ICC/IF 1:50 Human
FC 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

GAPDH is a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). GAPDH is usually used as a loading control for Western blot.

Cellular location

Cytoplasm, Cytoskeleton, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)
Lysate: 1: HeLa 2: NIH/3T3, 3: PC-12
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)
Lysate: 1: Arabidopsis
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 36 kDa
Observed band size: 36 kDa
IHC-P

Tissue: Human breast cancer
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6684

Tissue: Human pancreas
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6684

Tissue: Mouse colon
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6684

Tissue: Rat spleen
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature .
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6684
ICC/IF

Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-6684
FC

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:50
Secondary Ab: Goat Anti-Mouse IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies(Black line).
Isotype control: Mouse monoclonal IgG2b(Blue line).
Description: Line red is the positive signal for PTM-6684

Research Use

For research use only, not for use in diagnostic procedures.

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