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Recombinant Rabbit Monoclonal
Anti-GAPDH Rabbit mAb
Catalog Number: PTM-5375
$ 130

Clone Number: JRMR-10205-2

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF IP

Reactivity: Human, Monkey

Synonyms: GAPD

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms GAPD
UniProt ID

P04406

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:5000 Human, Monkey
IHC-P 1:50 - 1:100 Human
ICC/IF 1:50 Human
IP 1:25 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis.

Cellular location

Cytoplasm, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HepG2, 293T, SH-SY5Y, C2C12, COS-7, Human liver
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted band size: 36 kDa
Observed band size: 36 kDa
IHC-P

Tissue: Human pancreas
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5375
ICC/IF

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5375
IP

IP of HeLa cells extracts
IP Ab incubation: PTM-5375, 4°C overnight, 1:100 dilution
WB Primary Ab incubation: PTM_x0002_5375, room temperature 2h, 1:5000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 5% Input
2: IP with Rabbit monoclonal IgG Isotype
3: IP with PTM-5375
Observed band size: 36 kDa
Exposure time: 30 s

Research Use

For research use only, not for use in diagnostic procedures.

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References (5)
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    Authors Hejiang Guo, et al.
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  • GCN5 mediates DNA-PKcs crotonylation for DNA double-strand break repair and determining cancer radiosensitivity
    Year 2024
    Journal BRITISH JOURNAL OF CANCER
    Authors Han Yang, et al.
    Applications Unspecified
    Reactivity
  • Paradoxical expression of NRP1 in decidual stromal and immune cells reveals a novel inflammation balancing mechanism during early pregnancy
    Year 2023
    Journal INFLAMMATION RESEARCH
    Authors Chen Jiajia, et al.
    Applications Unspecified
    Reactivity
  • hsa_circ_0005991 promotes epithelial-mesenchymal transition by regulating miR-30b-3p/Cdc42EP1 axis in ovary endometriosis
    Year 2023
    Journal GENOMICS
    Authors Xiaoxu Liu, et al.
    Applications Unspecified
    Reactivity
  • Weighted gene co-expression network analysis identifies dysregulated B-cell receptor signaling pathway and novel genes in pulmonary arterial hypertension.
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