Background
Green fluorescent protein (GFP) was first identified as a key component of bioluminescence in the jellyfish Aequorea victoria. Composed of 238 amino acid residues with a molecular weight of 26.9kDa, GFP emits green fluorescence with an emission peak at 509 nm when excited by blue light (excitation peak at 395 nm. GFP has been extensively used in expression vectors as a fusion protein tag to facilitate the detection, localization, and real-time tracking of heterologous proteins in living systems.
Cellular location
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