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Anti-Glutaryllysine Mouse mAb
Catalog Number: PTM-1152
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB ChIP

Reactivity: All

Synonyms: Kglu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kglu
UniProt ID

/

Immunogen Glutarylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Glutaryllysine Mouse mAb detects proteins post-translationally modified by glutarylation on lysine residues. This pan antibody recognizes glutarylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Lysine glutarylation is a histone post-translational modification (PTM) that has been recently identified through integrated proteomic approaches and elaborate biochemistry analyses. This modification has been shown to be evolutionarily conserved across multiple species of prokaryotes and eukaryotes, and is present in a wide range of proteins, including both histones and non-histone substrates. Lysine glutarylation is a PTM that is regulated by SIRT5 and nutrient availability, and it has been demonstrated to impact various metabolic processes and mitochondrial functions.

Cellular location

/

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Immunogen peptide quantity: 1 ng, 4 ng, 16 ng Exposure time: 60 s The list of peptides is included in the table below: No. Peptide No. Peptide Lane 1 Kglu library Lane 2 Glutarylated BSA Lane 3 Succinylated BSA Lane 4 Malonylatd BSA Lane 5 BSA
WB

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysates: HeLa, N2a, BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: Multiple
Observed MW: Multiple

Research Use

For research use only, not for use in diagnostic procedures.

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References (3)
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