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Anti-Glutaryllysine Mouse mAb
Catalog Number: PTM-1152
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB

Reactivity: All

Synonyms: Kglu

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kglu
UniProt ID

/

Immunogen Glutarylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Glutaryllysine Mouse mAb detects proteins post-translationally modified by glutarylation on lysine residues. This pan antibody recognizes glutarylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Lysine glutarylation is a histone post-translational modification (PTM) that has been recently identified through integrated proteomic approaches and elaborate biochemistry analyses. This modification has been shown to be evolutionarily conserved across multiple species of prokaryotes and eukaryotes, and is present in a wide range of proteins, including both histones and non-histone substrates. Lysine glutarylation is a PTM that is regulated by SIRT5 and nutrient availability, and it has been demonstrated to impact various metabolic processes and mitochondrial functions.

Cellular location

/

Images
Dot Blot

eptide Lane 1 Kglu library Lane 2 Glutarylated BSA Lane 3 BSA Lane 4 BSA Lane 5 BSA
Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides and proteins used in the experiment is provided below.
Lane 1: Glutarylated peptide library. Lane 2: Glutarylated BSA.
Lane 3: Succinylated BSA. Lane 4: Malonylatd BSA.
Lane 5: BSA.
WB

Lysates: HeLa, N2a, and BRL cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Research Use

For research use only, not for use in diagnostic procedures.

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References (3)
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  • SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching
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