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Recombinant
Anti-Glutathione Synthetase Mouse mAb
Catalog Number: PTM-5695
$ 280

Clone Number: 11653

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: GSS

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1/kappa
Conjugate Unconjugated
Synonyms GSS
UniProt ID

P48637

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
IHC-P 1:50 - 1:100 Human, Mouse, Rat
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.

Cellular location

Cytosol

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 1: Human liver, 2: Mouse liver, 3: Rat liver
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 52 kDa
Observed band size: 52 kDa

IHC-P

Tissue: Human liver cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5695

Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5695

Tissue: Rat liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5695

Research Use

For research use only, not for use in diagnostic procedures.