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Recombinant Rabbit Monoclonal
Anti-HA Tag Rabbit mAb
Catalog Number: PTM-5389
$ 130

Clone Number: JRMR-10036-1

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF IP ChIP

Reactivity: /

Synonyms: /

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms /
UniProt ID

/

Immunogen /
MW (kDa) 50
Specificity Anti-HA Tag Rabbit mAb recognizes the HA tag sequence and is designed to detect exogeneously expressed HA-tagged proteins.
Product Usage Information
Applications Dilution Recommended Species
WB 1:5000 - 1:10000 /
ICC/IF 1:1000 - 1:2000 /
IP 1:25 /
ChIP 6 μg/2x106 cells /
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Tag antibody is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins.

Cellular location

/

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000/1:5000/1:10000
Primary Ab incubation: 2 hours at room temperature
Lysate: 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate ; 2: HEK-293T transfected with TIM4 (WT) (50 kDa) expression vector containing a HA-tag, whole cell lysate
Protein loading quantity: 10 μg
Exposure time: 60 s
Predicted band size: 42 kDa
Observed band size: 50 kDa
IP

IP of HEK-293T transfected with TIM4 (WT)
(50 kDa) expression vector containing a HA_x0002_tag, whole cell lysate
IP Ab incubation: PTM- 5389, 4°C overnight, 1:25 dilution
WB Primary Ab incubation: PTM5389, room temperature 2h, 1: 1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 5% Input
2: IP with PTM-5389
3: IP with Rabbit monoclonal IgG Isotype
Observed band size: 50 kDa
Exposure time: 120 s
ICC/IF

Cell line: 293T transfected with Control plasmid
Fixation: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:2000
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for PTM-5389

Cell line: 293T transfected with HA tag plasmid
Fixation: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:2000
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for PTM-5389
ChIP

Cell type: 293T transfected with HA-TEST (20
kDa) expression vector containing a HA-tag
Cross-linking conditions: Cells were fixed
with EGS for 30 minutes, then formaldehyde for
10 minutes.
Amount of chromatin per IP: 2×106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of
Protein A MagBeads
Comment:
The ChIP was performed with 6 μg of normal R
abbit IgG as a negative control. Real time
quantitative PCR was performed on
immunoprecipitated DNA using primers specific
for the human DHX40 promoter, CLTC promoter,
TTC16 promoter, FLYWCH2 promoter and
HES1 intron 2. Data are presented as
enrichment of each sample relative to total
amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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    Catalog #
    PTM-5177
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    /
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (4)
  • Dual-site molecular glues for enhancing protein-protein interactions of the CDK12-DDB1 complex
    Year 2024
    Journal Nature Communications
    Authors Zhang Zemin, et al.
    Applications Unspecified
    Reactivity
  • Fraxinellone alleviates colitis-related intestinal fibrosis by blocking the circuit between PD-1+ Th17 cells and fibroblasts
    Year 2024
    Journal INTERNATIONAL IMMUNOPHARMACOLOGY
    Authors Yuejie Xu, et al.
    Applications Unspecified
    Reactivity
  • Isobaric tags for relative and absolute quantification-based proteomic analysis of host-pathogen protein interactions in the midgut of Aedes albopictus during dengue virus infection.
    Year 2022
    Journal Frontiers in Microbiology
    Authors Wang J, et al.
    Applications Unspecified
    Reactivity
  • Isobaric tags for relative and absolute quantification-based proteomic analysis of host-pathogen protein interactions in the midgut of Aedes albopictus during dengue virus infection.
    Year 2022
    Journal Frontiers in Microbiology
    Authors Jiatian Wang, et al.
    Applications Unspecified
    Reactivity
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