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Anti-Histone H2A.X Mouse mAb
Catalog Number: PTM-1005
$ 250

Clone Number: 2H8

Host: Mouse Clonality: Monoclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: Histone H2AX, H2a/x, H2AX, H2AFX

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Histone H2AX, H2a/x, H2AX, H2AFX
UniProt ID

P16104

Immunogen Synthetic peptide corresponding to the C-terminus of human histone H2A.X protein
MW (kDa) 15
Specificity Anti-Histone H2A.X Mouse mAb detects endogenous levels of histone H2A.X protein. It does not cross-react with other histone or non-histone proteins.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Among core histones, the H2A family shows the highest sequence divergence, especially in the C-terminal region, leading to variants including H2A.Z, H2A.X, MacroH2A, H2A.B etc. H2A histone family member X (H2A.X) is a histone H2A variant encoded by the H2AFX gene. H2A.X contributes to nucleosome-formation, chromatin-remodeling and DNA double-strand break repair and is considered as a tumor suppressor. An early cellular response to DNA damage is the rapid phosphorylation of H2A.X at position Ser139.

Cellular location

Nucleus

Images
WB

Lysates: MCF-7, HeLa, H1299, JK
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Lysates: (-) HeLa; (+) HeLa cells treated with 100 mg/ml nocodazole for 18 hours and 100 nM calyculin A for 1 hour
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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