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Recombinant Rabbit Monoclonal
Anti-Histone H3 Rabbit mAb (HRP Conjugate)
Catalog Number: PTM-6621
$ 300

Clone Number: 8-H2L4

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate HRP
Synonyms Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d
UniProt ID

P68431

Immunogen Synthetic peptide corresponding to the N-terminus of human histone H3 protein
MW (kDa) 15
Specificity Anti-Histone H3 Rabbit mAb (HRP Conjugate) detects endogenous levels of total histone H3 protein. It does not cross-react with other histone or non-histone proteins.
Product Usage Information
Applications Dilution Recommended Species
WB 1:5000-1:30000 Human, Mouse, Rat
IHC-P 1:100 - 1:500 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:30000
Primary Ab incubation condition: 2 hours at room temperature
Lysate: 1: HeLa, 2: HEK-293, 3: NIH/3T3, 4: PC-12, 5: Mouse brain, 6: Rat brain
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
IHC-P

Tissue: Human kidney
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6621

Tissue: Human testis
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6621

Tissue: Mouse colon
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6621

Tissue: Mouse liver
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6621

Tissue: Rat cardiac muscle
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6621

Tissue: Rat cerebrum
Section type: Formalin fixed & Paraffin - embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6621

Research Use

For research use only, not for use in diagnostic procedures.

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