Applications：
WB
IHC-P
ICC/IF
FC
IAP
IP
ChIP
mIHC-P
Clone Number: /
Host: Rabbit Clonality: Polyconal
Applications： WB ChIP
Reactivity: Human, Mouse, Rat, Monkey
Synonyms: Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d
Shipping: Ambient temperature
Order online or send purchase order to info@ptmbio.com
| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d |
| UniProt ID | |
| Immunogen | Synthetic peptide corresponding to the N-terminus of human histone H3 protein |
| MW (kDa) | 15 |
| Specificity | Anti-Histone H3 Rabbit pAb detects endogenous levels of total histone H3 protein. It does not cross-react with other histone or non-histone proteins. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:2000 | Human, Mouse, Rat, Monkey |
| ChIP | 6 μg per 5x106 cells | Human |
| Purity | Protein A and immunogen affinity purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Among the histone members, histone H3 is the most extensively modified and its amino-terminal tail structure has been shown to be especially critical for eukaryotic cells.
Cellular location
Nucleus
| WB | |
|---|---|
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lysates: HeLa, C2C12, COS |
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Lysates: (-) Hela; (+) Hela cells treated with 30 mM sodium butyrate for 4 hours |
| ChIP | |
|---|---|
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Cell type: HeLa |
Research Use
For research use only, not for use in diagnostic procedures.
