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Recombinant
Anti-HP1 alpha Mouse mAb
Catalog Number: PTM-7188
$ 280

Clone Number: 10184

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: Chromobox protein homolog 5, CBX5

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2b
Conjugate Unconjugated
Synonyms Chromobox protein homolog 5, CBX5
UniProt ID

P45973

Immunogen Peptide. This information is proprietary to PTMab.
MW (kDa) 23
Specificity /
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: MCF-7, 3: A431
Protein loading amount: 20 μg
Exposure time: 30 s
Predicted MW: 22 kDa
Observed MW: 23 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 290
    Recombinant Rabbit Monoclonal
    Anti-HP1 alpha Rabbit mAb
    Catalog #
    PTM-6213
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P