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Anti-HSP90 beta Rabbit pAb
Catalog Number: PTM-6970
$ 310

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: HSP 90, HSP90AB1, HSP90B, HSPC2, HSPCB

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms HSP 90, HSP90AB1, HSP90B, HSPC2, HSPCB
UniProt ID

P08238

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:2000 Human, Rat
IHC-P 1:50 - 1:100 Human, Mouse, Rat
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

HSP90 beta, also known as HSP90AB1, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers.

Cellular location

Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate)
Lysate: 1: HeLa, 2: K562, 3: Jurkat, 4: PC-12
Protein loading amount: 20 μg
Exposure time: 3 s
Predicted band size: 85 kDa
Observed band size: 90 kDa

IHC-P

Tissue: Human testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6970

Tissue: Mouse cerebrum
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6970

Tissue: Rat cerebrum
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6970

Research Use

For research use only, not for use in diagnostic procedures.

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